We narrowed to 2,459 results for: GEM

1. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

   Plasmid

   #110302

   Purpose

   Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

   Depositor

   Chris Kok-Lung Chan

   Article

   Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. TVBB C-term-mScarlet-Blast

   Plasmid

   #169221

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mScarlet-P2A-Blast
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CaMKII-somBiPOLES-mCerulean

   Plasmid

   #154948

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Has Service

   AAV Retrograde, AAV5, and AAV9

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa(0.4)

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2R3a-VenusYFP-3AT

   Plasmid

   #71269

   Purpose

   Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   VenusYFP
   

   Use

   Gateway

   Tags

   4xGly linker and T3A pea Pisum sativum ribulose-1…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. TVBB C-term-mScarlet-Puro

   Plasmid

   #169220

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mScarlet-P2A-Puro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMMK ENTR 4 CMV GST mTagBFP

   Plasmid

   #206259

   Purpose

   ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   GTS mTagBFP
   

   Use

   Multimate/gateway entr 4

   Tags

   BFGT1 (Golgi localisation peptide)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

   Plasmid

   #118274

   Purpose

   Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

   Depositor

   Dietmar Schmitz

   Article

   Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

   Inserts

   bPAC(WT)
   SthK
   

   Use

   AAV

   Tags

   His tag, P2A, mCherry, and myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CamK II

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMMACE DEST CMV H2B iRFP

   Plasmid

   #206253

   Purpose

   DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B iRFP713
   

   Use

   Recombinant baculovirus production, multimate/gat…

   Tags

   iRFP713

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TVBB C-term-mNeongreen-Puro

   Plasmid

   #169228

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mNeongreen-P2A-Puro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TVBB C-term-mTurquoise2-Hygro

    Plasmid

    #169225

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mTurquoise2-P2A-Hygro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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