We narrowed to 3,920 results for: pCMV mammalian

1. xCas9 3.7

   Plasmid

   #108379

   Purpose

   Mammalian expression vector for xCas9 3.7

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.7
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Equalizer-L plasmid

   Plasmid

   #169732

   Purpose

   Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Equalizer-M plasmid

   Plasmid

   #169731

   Purpose

   Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Equalizer-H plasmid

   Plasmid

   #169367

   Purpose

   Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. xCas9 3.6

   Plasmid

   #108384

   Purpose

   Mammalian expression vector for xCas9 3.6

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.6
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF-ABEmax

   Plasmid

   #164415

   Purpose

   EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   ABEmax
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

   Plasmid

   #228726

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D56R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

   Plasmid

   #228725

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

   Plasmid

   #228729

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R

    Plasmid

    #228730

    Purpose

    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D56R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PE_CO

    Plasmid

    #190335

    Purpose

    Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

    Depositor

    Rasmus Bak

    Article

    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

    Insert

    PE2 with codon-optimized reverse transcriptase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

    Plasmid

    #228735

    Purpose

    Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEJS2713-CMV-iNme2-ABE8e-i1-L10

    Plasmid

    #228734

    Purpose

    Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid iNme2 nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. evoBxb1

    Plasmid

    #222338

    Purpose

    Plasmid for expressing evolved Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

    Plasmid

    #176270

    Purpose

    Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    TadA-TadA*(8e)-dCasMINI-V4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. VRQR-ABEmax

    Plasmid

    #119811

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST-FLAG

    Plasmid

    #183511

    Purpose

    Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

    Plasmid

    #179686

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T1413I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge