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mTFP1-H3.3-N-14

Plasmid

#55490

Purpose

Localization: Nucleus/Histones, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

H3.3 (H3f3b Mouse)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCAG coca-GlyR-IRES-GFP

Plasmid

#242194

Purpose

Expression vector for cocaine-gated chemogenetic anion channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

coca-GlyR-IRES-GFP

Expression

Mammalian

Mutation

L141G G175K Y217F

Promoter

CAG

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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TOMM70-FKBP12-Halo donor plasmid

Plasmid

#239867

Purpose

endogenously tags TOMM70 with Halo tag

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TOMM70 (TOMM70 Human)

Use

Donor plasmid

Tags

Halo Tag

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbCas12a-EGFP-NLS-3xFlag

Plasmid

#236364

Purpose

Overexpression of dLbCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dLbCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-LacI-NUT-KS

Plasmid

#238279

Purpose

For overexpression of CFP-LacI-NUT-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI-NUT-KS

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-LacI-NUT-KS-FtoG

Plasmid

#238280

Purpose

For overexpression of CFP-LacI-NUT-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI-NUT-KS-FtoG

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-LacI-NUT

Plasmid

#238278

Purpose

For overexpression of CFP-LacI-NUT

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI-NUT

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DEST (JDW 471)

Plasmid

#242573

Purpose

Gateway destination vector with a CAGGS promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway subcloning

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLD1112 TRE-NLS-mMaroon1-NLS-22Xm6A MS2

Plasmid

#235129

Purpose

mMaroon1 reporter plasmid for live cell visualization of RNA m6A modification

Depositor

Qin Peng

Article

Li et al Sci Adv. 2024 Nov 15;10(46):eadp5689. do

Insert

TRE-NLS-mMaroon1-NLS-22Xm6A MS2

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLD1112 TRE-mCherry-SKL-22Xm6A MS2

Plasmid

#235128

Purpose

mCherry reporter plasmid for live cell visualization of RNA m6A modification

Depositor

Qin Peng

Article

Li et al Sci Adv. 2024 Nov 15;10(46):eadp5689. do

Insert

TRE-mCherry-SKL-22Xm6A MS2

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP14-1535-1601-Hygro

Plasmid

#238100

Purpose

Expression of structurally predicted WWE domain of PARP14 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP14 (PARP14 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1535-1601 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-97)-EGFP

Plasmid

#196230

Purpose

EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged DTX2(8-97)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(WT)-EGFP

Plasmid

#196233

Purpose

EGFP fused to the C-terminus of Af1521 (WT) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(WT)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(G42E)-myc

Plasmid

#196238

Purpose

Af1521(G42E) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

Af1521(G42E)-myc

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RNF146(92-168)-EGFP

Plasmid

#196228

Purpose

EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(92-168)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-48xHSPA1B array

Plasmid

#236378

Purpose

Expression of array of 48 crRNAs targeting HSPA1B

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xHSPA1B crRNA array (HSPA1B Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-48xNFIL3 array

Plasmid

#236379

Purpose

Expression of array of 48 crRNAs targeting NFIL3

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xNFIL3 crRNA array (NFIL3 Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-48xS100A10 array

Plasmid

#236381

Purpose

Expression of array of 48 crRNAs targeting S100A10

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xS100A10 crRNA array (S100A10 Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag

Plasmid

#236375

Purpose

Overexpression of dFnCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dFnCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236363

Purpose

Overexpression of endAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

endAsCas12a

Use

CRISPR and Lentiviral

Mutation

E174R, S542R, K548R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236362

Purpose

Overexpression of HyperdAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdAsCas12a

Use

CRISPR and Lentiviral

Mutation

E174R, D256R, E321R, E371R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236361

Purpose

Overexpression of dAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dAsCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-Ace-mNeon2-WPRE

Plasmid

#240056

Purpose

Cre dependent Green fluorescent, negative response-polarity voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

Ace-mNeon2

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-NPM1-repair-template for U2OS

Plasmid

#238246

Purpose

For knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-NPM1-repair-template for HCT116

Plasmid

#238245

Purpose

For knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_EGFP-BRD4-NUT-KS-FtoG

Plasmid

#238256

Purpose

For overexpression of EGFP-BRD4-NUT-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-BRD4-NUT-KS-FtoG

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A-KS

Plasmid

#238290

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A-KS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-YAP-MAML2-KS

Plasmid

#238276

Purpose

For overexpression of mEGFP-YAP-MAML2-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mEGFP-YAP-MAML2-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A

Plasmid

#238289

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_EGFP-BRD4-NUT-KS

Plasmid

#238255

Purpose

For overexpression of EGFP-BRD4-NUT-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-BRD4-NUT-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge