We narrowed to 4,734 results for: Drosophila

1. pUb FLAG-Mts H241A

   Plasmid

   #208404

   Purpose

   Expresses FLAG-tagged Drosophila mts protein with H241A mutation

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   microtubule star (mts Fly)
   

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   H241A

   Promoter

   Ubi-p63e

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUb FLAG-Mts

   Plasmid

   #195065

   Purpose

   Expresses FLAG-tagged Drosophila mts protein

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   microtubule star (mts Fly)
   

   Tags

   3xFLAG

   Expression

   Insect

   Promoter

   Ubi-p63e

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnEA-vH_His-TEV-DSep1-msfGFP

   Plasmid

   #174494

   Purpose

   bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Insert

   Sep1 (Sep1 Fly)
   

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MS2-HA-HsRoquin1_AE

   Plasmid

   #148611

   Purpose

   Mammalian Expression of HsRoquin1

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

   Insert

   HsRoquin1 (RC3H1 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MS2-HA-HsRoquin2_AE

   Plasmid

   #148614

   Purpose

   Mammalian Expression of HsRoquin2

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

   Insert

   HsRoquin2 (RC3H2 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 phiC31

   Plasmid

   #68310

   Purpose

   In vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).

   Depositor

   Konrad Basler

   Article

   Bischof et al Proc Natl Acad Sci U S A. 2007 Feb 27;10

   Insert

   phiC31
   

   Use

   Rna transcription

   Promoter

   CMV

   Available Since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBPnlsLexA::p65Uw

   Plasmid

   #26230

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   nlsLexA::p65 (RELA Human)
   

   Expression

   Insect

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWALIUM-intron 3x UAS attB

   Plasmid

   #206360

   Purpose

   includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

   Depositor

   Erdem Bangi

   Article

   Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEED

   Plasmid

   #219658

   Purpose

   Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Empty SEED Cassette (3xP3-dsRed)
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBPp65ADZpUw

    Plasmid

    #26234

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    p65AD-Zip (RELA Human)
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAT7

    Plasmid

    #204292

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Promoter

    3XP3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGE-attBTT-loxP-DsRed (pTL780)

    Plasmid

    #115160

    Purpose

    Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

    Depositor

    Tobias Langenhan

    Article

    Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

    Insert

    attB-TT loxP 3xP3-DsRed
    

    Use

    Cre/Lox

    Expression

    Insect

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBPLexA::p65Uw

    Plasmid

    #26231

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    LexA::p65 (RELA Human)
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEED-sfGFP

    Plasmid

    #219639

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH3U3-zif268 (omega)

    Plasmid

    #18046

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    zif268 binding site
    

    Expression

    Bacterial

    Mutation

    The preferred binding site for zif268 is inserted…

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

    Plasmid

    #205152

    Purpose

    Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    FRT-GFP-FRT-uORF-dCBEevoCDA1
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAST

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Su(fu) (CT#99)

    Plasmid

    #13856

    Depositor

    Cliff Tabin

    Article

    Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

    Insert

    Su(fu) (SUFU Chicken)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nco I fragment from full length; ~2kbp- ATG to st…

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

18. Ac5-STABLE2-puro

    Plasmid

    #208032

    Purpose

    Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUb PspCas13b + PspCas13b guide RNA

    Plasmid

    #176305

    Purpose

    Expresses PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E113K, N265S- please depositor comments

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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