We narrowed to 3,108 results for: N-EGFP

1. pmGFP-Sec16S

   Plasmid

   #15775

   Depositor

   Benjamin Glick

   Article

   Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

   Insert

   Sec16S (SEC16B Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]

   Plasmid

   #115893

   Purpose

   AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Has Service

   AAV8

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYO395

   Plasmid

   #235734

   Purpose

   Expression of human VPS34 complex I in mammalian cells

   Depositor

   Roger Williams

   Article

   Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

   Inserts

   ATG14 (ATG14 Human)
   EGFP
   BECN1 (BECN1 Human)
   PIK3C3 (PIK3C3 Human)
   PIK3R4 (PIK3R4 Human)
   

   Use

   Tags

   ZZ-3xTEV

   Expression

   Mammalian

   Mutation

   N256S

   Promoter

   CMV

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

   Plasmid

   #223106

   Purpose

   pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

   Plasmid

   #223107

   Purpose

   pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

   Plasmid

   #223108

   Purpose

   pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

   Plasmid

   #223109

   Purpose

   pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-mTagBFP2

   Expression

   Mammalian

   Mutation

   SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Thy1.1-G5

   Plasmid

   #234839

   Purpose

   pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

   Depositor

   Gabriel Victora

   Article

   Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

   Insert

   Thy1.1 (Thy1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-P2A-5xGly

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

   Plasmid

   #188778

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CARPID BASU-dCasRx

    Plasmid

    #153209

    Purpose

    express CARPID BASU-dCasRx fusion protein in mammalian cells

    Depositor

    Jian Yan, Liang Zhang

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    BASU-dCasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPC1000

    Plasmid

    #174829

    Purpose

    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Inserts

    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    EF1a and mouse U6

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TV-hPITX3-forward

    Plasmid

    #22073

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PITX3 (ASMD Human)
    

    Use

    Cre/Lox; Zfn donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. macroH2A2-GFP (pc2191)

    Plasmid

    #223709

    Purpose

    Expression of macroH2A2 tagged N-terminal to GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A2-GFP (MACROH2A2, H2AFY2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-CMV GFP-LC3B Q116P G120

    Plasmid

    #129290

    Purpose

    Expresses GFP-tagged deconjugation-resistant LC3B in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. cmamxA2-GFP

    Plasmid

    #107197

    Purpose

    Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    cmannexin A2 (ANXA2 Human)
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    all type II Ca2+ binding sites deleted ( D161A, E…

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpot-Tag-Lamin

    Plasmid

    #117062

    Purpose

    For mammalian expression of lamin B1 with N-terminal Spot-Tag

    Depositor

    Felix Hartlepp

    Insert

    Lamin B1 (LMNB1 Human)
    

    Use

    Tags

    Spot-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpot-Tag-Actin

    Plasmid

    #117060

    Purpose

    For mammalian expression of beta-actin with N-terminal Spot-Tag

    Depositor

    Felix Hartlepp

    Insert

    actin beta (ACTB Human)
    

    Use

    Tags

    Spot-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mini-YFP-hDF-C2F-TM

    Plasmid

    #72600

    Purpose

    mini human dysferlin (from domain F to transmembrane ) tagged with YFP

    Depositor

    Renzhi Han

    Article

    Cheng et al Nat Med. 2014 Oct;20(10):1187-92. doi: 1

    Insert

    dysferlin (DYSF Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    from domain F to transmembrane domain

    Promoter

    CMV

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

20. GFP-PK

    Plasmid

    #24163

    Depositor

    Warner Greene

    Article

    Sherman et al J Virol. 2001 Feb . 75(3):1522-32.

    Insert

    pyruvate kinase
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only