We narrowed to 2,459 results for: GEM

1. pMMK ENTR 2 CMV mKO2-hCdt1

   Plasmid

   #206282

   Purpose

   ENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMV

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   mKO2-hCdt1 (CDT1 Human)
   

   Use

   Multimate/gateway entr 2

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TVBB C-term-mTurquoise2-Puro

   Plasmid

   #169224

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2-P2A-Puro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV2_hSyn_iChloC_CA_Citrine

   Plasmid

   #98220

   Purpose

   Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iChloC_C128A gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   E83Q, E90R, E101S, C128A, T159C, D156N

   Promoter

   human synapsin

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV2_hSyn_Aurora_Citrine

   Plasmid

   #98217

   Purpose

   Red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Aurora gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

   Promoter

   human synapsin

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TVBB C-term-Dendra2-Puro

   Plasmid

   #169217

   Purpose

   Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked Dendra2-P2A-Puro
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(gKAT7-5)-PGKpuro2ABFP-W

   Plasmid

   #159287

   Purpose

   Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. 5)

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   guide RNA targeting KAT7 (ID. 5) (KAT7 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMMDS 2 colors

   Plasmid

   #206265

   Purpose

   Expression of 2 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   GST mTagBFP1 (Synthetic), Ctnnb1 (M. musculus) (Ctnnb1 )
   

   Use

   Cre-donor

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MultiMate-HITI-2c ACTB

   Plasmid

   #206267

   Purpose

   All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
   

   Use

   CRISPR; Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MultiMate-PE3 HEK3

   Plasmid

   #206273

   Purpose

   Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
   

   Use

   CRISPR; Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MultiMate-PE2 HEK3

    Plasmid

    #206274

    Purpose

    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    

    Use

    CRISPR; Recombinant baculovirus production, cre-a…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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