We narrowed to 4,734 results for: Drosophila

1. pSEED-mScarlet3

   Plasmid

   #219640

   Purpose

   Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRW-CRY2-VHH(GFP)

   Plasmid

   #126022

   Purpose

   express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

   Depositor

   Eurico Morais-de-Sá

   Article

   Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

   Insert

   CRY2 PHR-VHH(GFP)
   

   Tags

   RFP

   Expression

   Insect

   Mutation

   WT

   Promoter

   Hsp70

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAT71

   Plasmid

   #204305

   Purpose

   Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Promoter

   ie1

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEED-ALFA:HA

   Plasmid

   #219646

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   ALFA:HA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEED-sfGFP:ALFA

   Plasmid

   #219642

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP:ALFA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEED-mGreenLantern:ALFA

   Plasmid

   #219644

   Purpose

   Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mGreenLantern:ALFA SEED Cassette
   

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFlpStop-attB-UAS-2.1-tdTom

   Plasmid

   #88910

   Purpose

   attB FlpStop plasmid, for insertion into MiMIC site

   Depositor

   Tom Clandinin

   Article

   Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

   Insert

   FlpStop
   

   Expression

   Insect

   Promoter

   5XUAS

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT31

   Plasmid

   #204291

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-DsRed
   

   Use

   CRISPR

   Promoter

   ie1

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEED-mGreenLantern

   Plasmid

   #219643

   Purpose

   Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mGreenLantern SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBac-DsRed-ORCO_9kbProm-QF2

    Plasmid

    #104877

    Purpose

    Anopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoes

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Insert

    AgORCO-QF2
    

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEED-mScarlet3:ALFA

    Plasmid

    #219641

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet:ALFA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ninaE-norpA

    Plasmid

    #26854

    Depositor

    Craig Montell

    Article

    Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

    Insert

    no receptor potential A (norpA Fly)
    

    Expression

    Insect

    Mutation

    V963A

    Available Since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pSEED-mCherry

    Plasmid

    #219657

    Purpose

    Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPaint-3xP3-RFP

    Plasmid

    #130276

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    3xP3-RFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC-U63-gRNA2.1

    Plasmid

    #170512

    Purpose

    gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPaint-3xP3-GFP

    Plasmid

    #130277

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    3xP3-GFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LifeAct-MTED

    Plasmid

    #217498

    Purpose

    Expresses LifeAct-mScarlet-MTED for targeting fluorescent, MT depolymerizing peptide MTED to f-actin

    Depositor

    Erik Dent

    Article

    Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

    Insert

    LifeAct-mScarlet-MTED
    

    Expression

    Mammalian

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. UAS-mNAMPT

    Plasmid

    #187887

    Purpose

    Gal4/UAS expression of mammalian NAMPT

    Depositor

    Lukas Neukomm

    Article

    Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

    Insert

    mNAMPT
    

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPaint-sfGFP-3xP3-RFP

    Plasmid

    #127566

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sfGFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NCD-GFP

    Plasmid

    #24507

    Depositor

    Ron Vale

    Article

    Case et al Cell. 1997 Sep 5. 90(5):959-66.

    Insert

    NCD (ncd Fly)
    

    Tags

    GFP and His

    Expression

    Bacterial

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only