We narrowed to 6,754 results for: hwa

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p4xSMAD_RE

Plasmid

#118051

Purpose

GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

4 copies the SMAD DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

Plasmid

#98973

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 2 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pIMVAd39T

Plasmid

#98303

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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p2xp53_RE

Plasmid

#118053

Purpose

GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

2 copies of the p53 DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a T32A DBM (H212R)

Plasmid

#1793

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A, H212R

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

Plasmid

#99221

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.1

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

Plasmid

#83489

Purpose

cre-dependent PAK1-AID under EF1a promoter

Depositor

Dong Kong

Article

Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

Insert

PAK1-AID-ires-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag B)

Plasmid

#16606

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag B) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragB (KpnI->AccI). The KpnI site is at -1194 …

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
c-myc promoter (Frag C)

Plasmid

#16607

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag C) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragC (AccI->PvuII). The AccI site is at -741 …

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

Plasmid

#98972

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 1 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-StefinA

Plasmid

#182404

Purpose

Bacterial expression of Vesicle Nucleating peptide6-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide 6 (VNp6)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMX-Brn4

Plasmid

#47028

Purpose

Expresses mouse Brn4

Depositor

Hans Schöler

Article

Han et al Cell Stem Cell. 2012 Apr 6;10(4):465-72.

Insert

Brn4 (Pou3f4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

The insert includes 40 bp of the TOPO vector in t…

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only
ps226

Plasmid

#133557

Purpose

RNAi construct for knock-down of CDKN1A (Cip1, p21)

Depositor

David Livingston

Article

Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. do

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Plasmid

#204288

Purpose

yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Tags

mRuby3

Expression

Yeast

Promoter

pHis3

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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RSRC1

Plasmid

#155983

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RSRC1 (RSRC1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV mouse Vav1 IRES GFP

Plasmid

#107091

Purpose

Mammalian expression retroviral vector

Depositor

Steven Clifford, Martine Roussel

Article

Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 merlin 1-590

Plasmid

#11624

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pBS-mHopX

Plasmid

#16425

Depositor

Jonathan Epstein

Article

Chen et al Cell. 2002 Sep 20. 110(6):713-23.

Insert

Hopx (hop Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pBeast-pAhpC-RBS- sfGFP

Plasmid

#190051

Purpose

GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4beta

Plasmid

#15517

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

pCDNA3 Flag MKK4beta (MAP2K4 Human)

Tags

Flag

Expression

Bacterial

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only