We narrowed to 6,889 results for: nsf

1. pCMV NucleoBAS N354K-I372V

   Plasmid

   #63574

   Purpose

   Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

   Depositor

   Stan van de Graaf

   Article

   van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

   Insert

   NucleoBAS N354K-I372V
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   N345K and I372V

   Promoter

   CMV

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

2. mitoTALECD for_atp1_1397CN

   Plasmid

   #186203

   Purpose

   For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

   Inserts

   mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
   mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
   

   Use

   Synthetic Biology; Ti plasmid for plant transform…

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAR-9.47_VP2_AS

   Plasmid

   #74241

   Purpose

   Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV9.47-VP2_AS
   

   Use

   AAV

   Tags

   19 alanines fused to VP2 of AAV9.47

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. SopB-EGFP

   Plasmid

   #183655

   Purpose

   C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-ms-c-Myc-V394D

   Plasmid

   #50775

   Purpose

   retroviral expression of mouse c-Myc V394D mutant

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc V394D (Myc Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   V394D (does not bind to Miz-1)

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti HsATP10B - EmGFP

   Plasmid

   #204474

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B WT fused with EmGFP

   Depositor

   Veerle Baekelandt

   Article

   Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

   Inserts

   ATP10B (ATP10B Human)
   EmGFP
   

   Use

   Lentiviral

   Tags

   EmGFP

   Promoter

   CMV

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX131

   Plasmid

   #167149

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   4'-phosphopantetheinyl transferase, npgA
   

   Use

   Synthetic Biology

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE

   Plasmid

   #87919

   Purpose

   HIV-1 SIN lentiviral transfer vector

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   sgHTT1, shGFP2, sgCas9, cherry
   

   Use

   Lentiviral

   Promoter

   H1, U6, 7SK, PGK

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pESC-LEU-SopB

   Plasmid

   #183670

   Purpose

   Inducible Salmonella Typhimurium SopB for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Salmonella enterica serovar Typhimurium)
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pESC-Leu-SopB(C460S)

    Plasmid

    #183671

    Purpose

    Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Expression

    Yeast

    Mutation

    C460S

    Promoter

    GAL1

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-MRPP3-His

    Plasmid

    #67866

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP3 (KIAA0391 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-hu-c-Myc-dN2

    Plasmid

    #50774

    Purpose

    retroviral expression of human c-Myc dN2 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc -dN2 (MYC Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 42-439

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-hu-c-Myc-dN1

    Plasmid

    #50773

    Purpose

    retroviral expression of human c-Myc dN1 fragment

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    c-Myc -dN1 (MYC Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    N-terminal deletion, contains aa 14-439

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-)-V5.hOr47a

    Plasmid

    #126474

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI-neo-FHM

    Plasmid

    #51871

    Purpose

    Expresses N-terminal FLAG-HA-tagged full length rat MIBP1 (cloned into pCI-neo)

    Depositor

    Tomoko Tahira, Kenshi Hayashi

    Article

    Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

    Insert

    MIBP1 (Hivep2 Rat)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pS6-C-hα2M-H6

    Plasmid

    #128497

    Purpose

    Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (F789-S1468) (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid

    #133793

    Purpose

    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    CjeCas9 sgRNA entry vector
    

    Expression

    Mammalian

    Mutation

    V2-TGAA linker sgRNA architecture from Kim et al.…

    Promoter

    U6

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. His6-SMT3-SopB

    Plasmid

    #183676

    Purpose

    Recombinant Salmonella Typhimurium SopB

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
    

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. gRNAs[bTub+Sxl].1046B

    Plasmid

    #112692

    Purpose

    express two gRNA targeting bTub & Sxl under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST47-ARF4-GFP

    Plasmid

    #67392

    Purpose

    Mammalian expression of hArf4 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V53A, L123P and M134I mutations were unintended P…

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only