We narrowed to 14,430 results for: nts

1. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

   Plasmid

   #138525

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   Control non-PRC2 binding A-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDF-casBCDE(g8)

   Plasmid

   #89727

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.

   Depositor

   Konstantin Severinov, Konstantin Kuznedelov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_SNORD27h_2ntmut

   Plasmid

   #73067

   Purpose

   expression clone for human mutated SNORD27 (C/D box snoRNA U27)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD27 (E2F7 Human)
   

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   2 nt mutation in AS box that binds E2F7 pre-mRNA

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR

   Plasmid

   #118622

   Purpose

   expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons

   Depositor

   Marina Chekulaeva

   Article

   Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

   Insert

   cdc42E7-E6 3'UTR (Cdc42 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   changed ttgcttt to cggtaag (496 - 502 nt in 3…

   Promoter

   synapsin I (rat)

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMEXneo-TrkC-Δ6

   Plasmid

   #190204

   Purpose

   To express the human TrkC that has in the extracellular domain only the second IgG like domain. Still binds NT-3.

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

   Insert

   TrkC (NTRK3 Human)
   

   Expression

   Mammalian

   Mutation

   The extracellualr domain conatins only the signal…

   Promoter

   Moloney mouse sarcoma virus right LTR

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUb FLAG-tws

   Plasmid

   #195068

   Purpose

   Expresses FLAG-tagged Drosophila tws protein

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   twins (tws Fly)
   

   Tags

   3xFLAG

   Expression

   Insect

   Promoter

   Ubi-p63e

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRosettav2

   Plasmid

   #115557

   Purpose

   To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments

   Depositor

   John Doench

   Insert

   Hygromycin-Blasticidin-Puromycin-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-ePEG-LINE1-loxPsym

   Plasmid

   #216866

   Purpose

   Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

   Depositor

   Leopold Parts

   Insert

   spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET.HaloTag.v7.CoOp.Cys-less

   Plasmid

   #195487

   Purpose

   Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C61T, C262V

   Promoter

   T7

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDR195

    Plasmid

    #36028

    Depositor

    Wolf Frommer, Dominique Loque

    Article

    Rentsch et al FEBS Lett. 1995 Aug 21;370(3):264-8.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    pPMA1

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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