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pAAV asyn S87A

Plasmid

#36056

Depositor

Hilal Lashuel

Article

Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

Insert

alpha-synuclein S87A (SNCA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

S87A

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-uTEV1-MAVS-mTA4

Plasmid

#171002

Purpose

HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIVLI]

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1996

Plasmid

#49126

Purpose

AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple267-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

UGT8

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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FKBP-Stx3-mCitrine

Plasmid

#92428

Purpose

Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Use

Tags

FKBP (FK506 binding protein 12); allows for heter…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_GABPA

Plasmid

#64253

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPA

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GABPA Homology Arms (GABPA Human)

Use

Tags

3X Flag P2A Neo

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEMS1977

Plasmid

#49109

Purpose

AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple251-icre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

C8ORF46

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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FKBP-Stx4-mCitrine

Plasmid

#92427

Purpose

Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx4 (Stx4 Rat)

Use

Tags

FKBP (FK506 binding protein 12); allows for heter…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-FAM86A

Plasmid

#85117

Purpose

bacterial expression of FAM86A

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EEF2KMT (FAM86A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Mutant Human Cortactin-GFP

Plasmid

#71365

Purpose

mammalian expression of mutant human Cortactin-GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

cortactin (CTTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

silent mutation present at 72nd nucleotide

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only
pLV.5’VIM-eGFP-KI

Plasmid

#170547

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for VIM knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 FS10

Plasmid

#113956

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, S212N substitution…

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-gfap-sgRNA

Plasmid

#65566

Purpose

in vitro trancription of sgRNA targeting the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap sgRNA (gfap Zebrafish)

Use

In vitro rna transcription

Tags

Expression

Mutation

Promoter

T7

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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eGFP-KCC2

Plasmid

#104075

Purpose

expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

Depositor

Igor Medyna

Article

Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

CMV

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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CIAR_pcDNA5/FRT/TO

Plasmid

#86502

Purpose

Expresses CIAR in mammalian cells. Used to generate Flp-In stables.

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CIAR (SOS1 Human)

Use

Frt

Tags

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV (tet operator)

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

Plasmid

#113952

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, I132S and S212N su…

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2157

Plasmid

#70118

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-EmGFP-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pFETCh_KMT2B

Plasmid

#101070

Purpose

Donor vector for 3' FLAG tag of human KMT2B

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KMT2B homology arms (KMT2B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST/N1-hEB1-GFP

Plasmid

#27382

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

microtubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
PX458_KMT2B_2

Plasmid

#101075

Purpose

Encodes gRNA for 3' target of human KMT2B

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KMT2B (KMT2B Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KMT2B_1

Plasmid

#101074

Purpose

Encodes gRNA for 3' target of human KMT2B

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KMT2B (KMT2B Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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