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Showing: 5761 - 5800 of 8357 results

  1. pQdCas12a.luxR(mut)-empty

    Plasmid
    #236037
    Purpose
    The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI, dcas12a, and lacZ-alpha (fragment).
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236037-plasmid-map-sequence-id-469909

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  2. pCAGGS-â–³MTS-TFAM-V5

    Plasmid
    #200934
    Purpose
    expresses â–³MTS-TFAM-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    Transcription Factor A, Mitochondrial (TFAM Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    deleted mitochondrial targeting sequence (MTS)
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200934-plasmid-map-sequence-id-410627

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  3. pCAGGS-MTS(S17A)-TFAM-V5

    Plasmid
    #203668
    Purpose
    expresses MTS(S17A)-TFAM-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    Transcription Factor A, Mitochondrial (TFAM Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S17A
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203668-plasmid-map-sequence-id-409415

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  4. pCAGGS-MTS(S43A)-TFAM-V5

    Plasmid
    #203669
    Purpose
    expresses MTS(S43A)-TFAM-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    Transcription Factor A, Mitochondrial (TFAM Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S43A
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    203669-plasmid-map-sequence-id-409431

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  5. pCAGGS-FLAG-BubR1

    Plasmid
    #200814
    Purpose
    expresses BubR1 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200814-plasmid-map-sequence-id-410103

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  6. pAG426-GAL-EWSR1

    Plasmid
    #181725
    Purpose
    Galactose inducible expression of EWSR1
    Depositor
    Alejandro Chavez
    Insert
    EWSR1 (EWSR1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    181725-plasmid-map-sequence-id-466838

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  7. pWPI-Glis3M412

    Plasmid
    #216854
    Purpose
    This plasmid is for the production of lentiviral vectors expressing an active form of Glis3 together with GFP.
    Depositor
    Patrick Salmon
    Insert
    Glis3 (Glis3 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    N-terminal deletion removing repressor domains
    Promoter
    EF1 alpha
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    216854-plasmid-map-sequence-id-464607

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  8. pCAG-GluA2-Fab-HC-Halo

    Plasmid
    #230061
    Purpose
    Expresses heavy chain Fab fragment of anti-GluA2 fused to HaloTag. Can be used with light chain to produce Fab in HEK cells.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 Fab heavy chain fragment
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230061-plasmid-map-sequence-id-460113

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  9. pPMS-2397

    Plasmid
    #231044
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to chicken Fc
    Use
    Affinity Reagent/ Antibody
    Tags
    8xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231044-plasmid-map-sequence-id-457178

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  10. pNY-346

    Plasmid
    #231046
    Purpose
    Used for staining GFP
    Depositor
    Jeremy Nathans
    Insert
    anti GFP DARPin fused to human Fc
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231046-plasmid-map-sequence-id-458329

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  11. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Puromycin resistance cassette has silent mutation…
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

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  12. pDual_dsCas9_Puro_hs_TP73

    Plasmid
    #214689
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Puromycin resistance cassette has silent mutation…
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214689-plasmid-map-sequence-id-428791

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  13. pAcGFP-C1-DGKd2

    Plasmid
    #224897
    Purpose
    Express N-terminal mCherry fused to human diacylglycerol kinase delta isoform 2 in mammalian cells
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    AcGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    224897-plasmid-map-sequence-id-456312

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  14. EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

    Plasmid
    #226590
    Purpose
    Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.
    Depositor
    D. James Surmeier
    Insert
    PSAML-141F,Y115F-GlyR
    Use
    AAV
    Tags
    P2A-Fusion Red
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    226590-plasmid-map-sequence-id-450965

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  15. CUP1-∆ssCPY*-mGFP

    Plasmid
    #212197
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake, Chris MacDonald
    Insert
    Carboxypeptidase Y variant (PRC1 Budding Yeast)
    Use
    Tags
    mEGFP (monomeric version EGFP, A206K mutation)
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    CUP1
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    212197-plasmid-map-sequence-id-450955

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  16. pIND4-RC-C2-M210TAG+M252V-oCNF

    Plasmid
    #222995
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    Phe variants PylRs
    Mm Pyl tRNA for oF incorporation
    Use
    Tags
    6xHis
    Expression
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222995-plasmid-map-sequence-id-444743

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  17. Vector 1174K

    Plasmid
    #221017
    Purpose
    Fluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)
    Depositor
    Omar Akbari
    Insert
    Engineered dobulesex splicing module (Anopheles gambiae)
    Use
    Synthetic Biology
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    221017-plasmid-map-sequence-id-441587

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  18. N-SF-TAP-DEST-TLK2-D592V

    Plasmid
    #218835
    Purpose
    Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).
    Depositor
    Travis Stracker
    Insert
    TLK2 Q592V (kinase dead) (TLK2 Human)
    Use
    Tags
    FLAG-STREP
    Expression
    Mammalian
    Mutation
    Q592V
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    218835-plasmid-map-sequence-id-436652

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  19. pSLIK DNM1L-V5 neo

    Plasmid
    #220363
    Purpose
    Lentiviral expression vector for an inducible V5-tagged DNM1L construct
    Depositor
    Kevin Janes
    Insert
    DNM1L (DNM1L Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220363-plasmid-map-sequence-id-436888

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  20. 8TBX

    Plasmid
    #218228
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DDX1 (DDX1 Human)
    Use
    Baculovirus expression
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 17, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    218228-plasmid-map-sequence-id-430075

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  21. pET50_G495R∆2CARD

    Plasmid
    #216805
    Purpose
    Expresses a single point mutant of melanoma differentiation-associated protein 5 lacking its 2 CARD domains
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    G495R/∆1-287
    Promoter
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    216805-plasmid-map-sequence-id-428836

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  22. pLKO-Tet-On-ARNT-shRNA1

    Plasmid
    #166910
    Purpose
    Lentivirus for inducible knockdown of human ARNT
    Depositor
    Roland Friedel
    Insert
    ARNT shRNA (ARNT Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166910-plasmid-map-sequence-id-331201

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  23. pLKO-Tet-On-ARNT-shRNA2

    Plasmid
    #166911
    Purpose
    Lentivirus for inducible knockdown of human ARNT
    Depositor
    Roland Friedel
    Insert
    ARNT shRNA (ARNT Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166911-plasmid-map-sequence-id-414797

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  24. pLKO-Tet-On-ARNT-shRNA3

    Plasmid
    #166912
    Purpose
    Lentivirus for inducible knockdown of human ARNT
    Depositor
    Roland Friedel
    Insert
    ARNT shRNA (ARNT Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    166912-plasmid-map-sequence-id-414799

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  25. 8GCY

    Plasmid
    #214440
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBLB:PBC020-G02:C248501 (CBLB Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 11, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    214440-plasmid-map-sequence-id-424893

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  26. 8DYS

    Plasmid
    #214439
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EIF2A:PBC022-C06:C245159 (EIF2A Human)
    Use
    Baculovirus
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Feb. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    214439-plasmid-map-sequence-id-424355

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  27. pET28-eIF4E-TwStrep

    Plasmid
    #208638
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)
    Depositor
    Quanfu Ma
    Insert
    human eIF4E (ENST00000450253) (EIF4E Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208638-plasmid-map-sequence-id-415918

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  28. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  29. pGB-05-06-GST-ATG14

    Plasmid
    #187987
    Purpose
    Plasmid for the protein expression of GST-ATG14 in a bacterial and insect expression system. Internal_Reference: SMC1321
    Depositor
    Sascha Martens
    Insert
    ATG14 (ATG14 Human)
    Use
    Tags
    GST
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187987-plasmid-map-sequence-id-374440

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  30. human KANK2 Ankyrin Repeats pET151

    Plasmid
    #164054
    Purpose
    Expresses human KANK2 Ank domain in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164054-plasmid-map-sequence-id-327671

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  35. pB6-Hipp11-td-sfGFP-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183026
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-td-sfGFP-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183026-plasmid-map-sequence-id-360495

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  36. pB6-Hipp11-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183025
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183025-plasmid-map-sequence-id-360497

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  37. pB6-Hipp11-tdTomato cre reporter-FNF-DTA

    Plasmid
    #183024
    Purpose
    B6J Hipp11-pCAG-loxP-FNF-bGHpA-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183024-plasmid-map-sequence-id-360499

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  38. MDKA-c014

    Plasmid
    #175464
    Purpose
    Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143. Fused to IgG Fc.
    Depositor
    Opher Gileadi
    Insert
    MDK (MDK Human)
    Use
    Tags
    HRV 3C-Fc-His6 and PTPRS (Gg) SP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175464-plasmid-map-sequence-id-353255

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  39. EPB41L3A-c021

    Plasmid
    #175460
    Purpose
    Protein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).
    Depositor
    Opher Gileadi
    Insert
    EPB41L3 (EPB41L3 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175460-plasmid-map-sequence-id-353154

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Showing: 5761 - 5800 of 8357 results