We narrowed to 4,734 results for: Drosophila

1. pBFv-UAS3

   Plasmid

   #138399

   Purpose

   10xUAS expression vector with a vermilion marker and a phiC31 attB sequence

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   UAS

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEED-Dendra2

   Plasmid

   #219645

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Dendra2 SEED Cassette
   

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUb RxCas13d + RxCas13d guide RNA

   Plasmid

   #176303

   Purpose

   Expresses RxCas13d and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   RxCas13d
   

   Tags

   HA tag

   Expression

   Insect

   Promoter

   Ubi-p63e

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEED-OLLAS:HA

   Plasmid

   #219647

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   OLLAS:HA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUb dPspCas13b + PspCas13b guide RNA

   Plasmid

   #176306

   Purpose

   Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   PspCas13b
   

   Tags

   HA tag

   Expression

   Insect

   Mutation

   H133A, H1058A

   Promoter

   Ubi-p63e

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTV-Gal4DBD2xHighMag1

   Plasmid

   #182023

   Purpose

   Donor plasmid for conversion of Gal4 line into ShineGal4 drivers

   Depositor

   Jean-Paul Vincent

   Article

   di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

   Insert

   Gal4DBD2xHighMag1
   

   Use

   Donor plasmid

   Expression

   Insect

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAC-DsRed-QF2-hsp70

   Plasmid

   #104876

   Purpose

   General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

   Insert

   QF2
   

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31

   Plasmid

   #165887

   Purpose

   G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   All-in-one FC31 RMCE Cassette
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

   Plasmid

   #71811

   Purpose

   Generating transgenic flies for FLP-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
   

   Expression

   Insect

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pR6Kg-Alpha1

    Plasmid

    #165862

    Purpose

    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJFRC7-UAS-NPRR_ANP

    Plasmid

    #133243

    Purpose

    Fluorescent reporter for neuropeptide release imaging

    Depositor

    David Anderson

    Article

    Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

    Insert

    rat atrial natriuretic peptide-fused codon-optimized GCaMP6s
    

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHRW-CRY2olig-VHH(GFP)

    Plasmid

    #126023

    Purpose

    express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CRY2 PHRolig-VHH(GFP)
    

    Tags

    RFP

    Expression

    Insect

    Mutation

    CRY2 E490G

    Promoter

    Hsp70

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pR6Kg-Omega1

    Plasmid

    #165864

    Purpose

    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET16b-Antp

    Plasmid

    #86866

    Purpose

    Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

    Depositor

    Michael Chin

    Article

    Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

    Insert

    Linker-Antp
    

    Tags

    Antp

    Expression

    Bacterial

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ac5-STABLE2-blast

    Plasmid

    #208034

    Purpose

    Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCaSpeR-act5cB-GAL4QF

    Plasmid

    #61309

    Purpose

    Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    GAL4_DBD::QF2w_AD
    

    Expression

    Insect

    Mutation

    Changed EK on C-terminus of QF2 to KKKK

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. OA-1050G (white)

    Plasmid

    #132420

    Purpose

    express arrays of gRNA targeting White under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    white gRNA array
    

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQUAST-syb-spCFP1-10

    Plasmid

    #73699

    Purpose

    Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP

    Depositor

    Marco Gallio

    Article

    Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

    Insert

    Neuronal-synaptobrevin:spCFP1–10 chimera
    

    Expression

    Insect

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF3BGX

    Plasmid

    #138387

    Purpose

    Empty RMCE vector (phiC31 attB/loxP)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    P5' FRT3 3xP3-Venus φC31-attB loxP FRT3 P3'
    

    Expression

    Insect

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 907H1

    Plasmid

    #160234

    Purpose

    FlightN-CymR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    p-cymene repressor
    Flightin promoter
    

    Tags

    VP16

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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