We narrowed to 24,491 results for: Spr;

1. pYPQ-dpcoCas9-EV2.1

   Plasmid

   #158411

   Purpose

   CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_KanR neo

   Plasmid

   #167861

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

   Plasmid

   #68405

   Purpose

   The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
   FlpO
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Promoter

   Synthetic Probasin ARRx2 and U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. enAsCas12a-mSA

   Plasmid

   #182954

   Purpose

   Express enAsCas12a-mSA

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   enAsCas12a-mSA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. NAX_CAGGS_CAS9_EBFP2

   Plasmid

   #167857

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD8A-RGR-r10

   Plasmid

   #74370

   Purpose

   This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   RGR-r10
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB5191

   Plasmid

   #126911

   Purpose

   Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   X-4 and XII-5 gRNA
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRnc-gGFP161

   Plasmid

   #189536

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-161-GFP
   

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458_NFIA_iso1_2

   Plasmid

   #104049

   Purpose

   Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   NFIA_iso1 gRNA (NFIA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458_NFIA_iso1_1

    Plasmid

    #104048

    Purpose

    Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NFIA_iso1 gRNA (NFIA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTX198

    Plasmid

    #89262

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA01
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    Maize Ubiquitin1 promoter

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458_NFIL3_2

    Plasmid

    #86349

    Purpose

    Encodes gRNA for 3' target of human NFIL3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against NFIL3 (NFIL3 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_NFIL3_1

    Plasmid

    #86348

    Purpose

    Encodes gRNA for 3' target of human NFIL3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against NFIL3 (NFIL3 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEL666

    Plasmid

    #196814

    Purpose

    LrCas9 genome editing plasmid in rice

    Depositor

    Yong Zhang

    Article

    Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    ZmUbi

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMOD8A-iRGR-r11

    Plasmid

    #74369

    Purpose

    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r11
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    AHD1

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. NAX_CAGGS_CAS9_EYFP

    Plasmid

    #167858

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pColE1_sgRNA_M7NS_AmpR

    Plasmid

    #119157

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pColE1_sgRNA_M2S_AmpR

    Plasmid

    #119154

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas12j3 KRAB

    Plasmid

    #188507

    Purpose

    Expresses FLAG tagged dCas12j3 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12j3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D413A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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