We narrowed to 23,433 results for: Spr;

1. pUC19-hsg(NT:NT)

   Plasmid

   #138260

   Purpose

   Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   Nontargeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB1C3-FeGFP

   Plasmid

   #138261

   Purpose

   Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   eGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB1C3-ysg(NT)

   Plasmid

   #138263

   Purpose

   Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   Nontargeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX330_ZBTB7B_2

   Plasmid

   #135747

   Purpose

   Encodes gRNA for 3' target of human ZBTB7B

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB7B gRNA (ZBTB7B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PX330_ZBTB7B_1

   Plasmid

   #135746

   Purpose

   Encodes gRNA for 3' target of human ZBTB7B

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZBTB7B gRNA (ZBTB7B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

   Plasmid

   #117140

   Purpose

   Express gRNA vs Dmap1

   Depositor

   Kosuke Yusa

   Insert

   gDmap1 v1 (Dmap1 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPOR0SP0012

   Plasmid

   #117533

   Purpose

   Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   eSaCas9 (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   R499A,Q500K,R654A,G655R

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR0SP0019

   Plasmid

   #117529

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.1 (no stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K848A, K1003A, R1060A

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPOR0SP0018

   Plasmid

   #117528

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.1

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.1 (with stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   K848A, K1003A, R1060A

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEPOR0SP0017

    Plasmid

    #117527

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.0 (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K810A, K1003A, R1060A

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEPOR0SP0015

    Plasmid

    #117525

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-KA (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K855A

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPOR0SP0001

    Plasmid

    #117523

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-DE (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D1135E

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP

    Plasmid

    #114730

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M7
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_ZNF511_1

    Plasmid

    #104044

    Purpose

    Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF511 gRNA (ZNF511 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX458_ZNF511_2

    Plasmid

    #104045

    Purpose

    Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF511 gRNA (ZNF511 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTX203

    Plasmid

    #89267

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsDEP1-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTX202

    Plasmid

    #89266

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA01

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsDEP1-gRNA01
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTX200

    Plasmid

    #89264

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsYSA-gRNA01
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTX199

    Plasmid

    #89263

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Maize Ubiquitin1 promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJB172

    Plasmid

    #86992

    Purpose

    CRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1

    Depositor

    Julien Berro

    Article

    Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

    Insert

    gRNA targeting pil1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge