Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,952 results for: codon optimized

Clear keywords
Showing: 2881 - 2900 of 4952 results

  9. AAV2_hSyn_Phobos_CA_Citrine

    Plasmid
    #98218
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Publication
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Phobos_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…
    Promoter
    human synapsin
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98218-plasmid-map-sequence-id-256051

    Enlarge

  13. pHS1068

    Plasmid
    #205962
    Purpose
    NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Insert
    DinG HNH proteins and CRISPR array
    Expression
    Bacterial
    Promoter
    Lac (proteins), pJ23119 (crRNA)
    Available since
    Feb. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    205962-plasmid-map-sequence-id-421621

    Enlarge

  14. B-HiFi SpCas9

    Plasmid
    #207362
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-HiFi SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    R691A, amino acids 1005-1013 replaced with two gl…
    Promoter
    CBh
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207362-plasmid-map-sequence-id-410135

    Enlarge

  15. B-Sniper SpCas9

    Plasmid
    #207361
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207361-plasmid-map-sequence-id-410129

    Enlarge

  16. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Publication
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

    Enlarge

  17. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid
    #201022
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.
    Depositor
    Sharon Rozovsky
    Publication
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    201022-plasmid-map-sequence-id-394460

    Enlarge

  18. pEPQD1CB0202

    Plasmid
    #185626
    Purpose
    MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter
    Depositor
    Nicola Patron
    Publication
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    CRISPR and Synthetic Biology
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185626-plasmid-map-sequence-id-368553

    Enlarge

  19. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126777
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126777-plasmid-map-sequence-id-410581

    Enlarge

Showing: 2881 - 2900 of 4952 results