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We narrowed to 4,853 results for: codon optimized

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Showing: 2881 - 2900 of 4853 results

  1. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    155046-plasmid-map-sequence-id-308670

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  2. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

    Plasmid
    #197028
    Purpose
    AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Use
    AAV
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197028-plasmid-map-sequence-id-388968

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  3. pSL8055 (dCas12f–σE (sigmaE) system, native Fta guide)

    Plasmid
    #254271
    Purpose
    Encodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters with guide targeting native Fta gene
    Depositor
    Samuel H. Sternberg
    Publication
    Hoffmann et al Nature. 2026 Mar 4. doi: 10.1038/s41586-
    Inserts
    dCas12f
    sigma E
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23105
    Available since
    May 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    254271-plasmid-map-sequence-id-510028

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  6. pDL0_198

    Plasmid
    #210865
    Purpose
    C1_Pyrearinus termitilluminans beetle luciferase ('Eluc' version - human-codon optimized)
    Depositor
    Dmitri Nusinow
    Publication
    Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.
    Insert
    Pyrearinus termitilluminans beetle luciferase
    Use
    Luciferase and Synthetic Biology
    Expression
    Plant
    Available since
    June 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    210865-plasmid-map-sequence-id-421293

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  7. Sc++

    Plasmid
    #198545
    Purpose
    Expresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    Sc++
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198545-plasmid-map-sequence-id-388902

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  8. SpCas9-NRRH

    Plasmid
    #198540
    Purpose
    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRRH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198540-plasmid-map-sequence-id-388897

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  16. pMXs-IRES-blast GFP-SPNS1(E164K)

    Plasmid
    #198572
    Purpose
    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Publication
    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi
    Insert
    SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    Use
    Retroviral
    Tags
    3xFlag-GFP
    Expression
    Mammalian
    Mutation
    changed glutamic acid E164 to lysine (E164K)
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    198572-plasmid-map-sequence-id-438878

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  17. GB3668

    Plasmid
    #215243
    Purpose
    TU for the expression of mAID (N-tag): AcrIIA4 w/o NLS protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and TNOS terminator
    Depositor
    Diego Orzaez
    Publication
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    P35S:mAID:AcrIIA4:TNOS
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215243-plasmid-map-sequence-id-436560

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  20. P15-pZE-SUMO-PiTTA

    Plasmid
    #204630
    Purpose
    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized piTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
    Depositor
    Aditya Kunjapur
    Publication
    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:
    Insert
    His-SUMO-TEV-BuTTA
    Use
    Synthetic Biology
    Tags
    His Tag; SUMO Tag; TEV Protease Site
    Expression
    Bacterial
    Promoter
    PLtetO-1 promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204630-plasmid-map-sequence-id-414417

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Showing: 2881 - 2900 of 4853 results