We narrowed to 14,583 results for: pET

1. pET22b-T5-sfGFP*-XcP4H-MH4R

   Plasmid

   #178043

   Purpose

   Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

   Depositor

   Han Xiao

   Article

   Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

   Inserts

   superfolder green fluorescent protein with TAG at 151 position
   XcP4H with W179F mutation
   dihydropterine reductase from human
   pterin-4a-carbinolamine dehydratase from human
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28_mouse-Caspase-1_enzymatic-domain

   Plasmid

   #183385

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 (AA130-404) (Casp1 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b-ACPs (from S. pneumonia)

   Plasmid

   #63687

   Purpose

   Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae

   Depositor

   Michael Johnson

   Insert

   acpS
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET-6xHis-(pos36)GFP

   Plasmid

   #62937

   Purpose

   Expression of 6xHis-(+36)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (pos36)GFP
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-TS2126 RnlA-Strep

   Plasmid

   #159350

   Purpose

   Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

   Depositor

   Takashi Ito

   Article

   Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

   Insert

   TS2126 RNA ligase
   

   Use

   Tags

   His6 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a-GFP-AURKA_K162M-mCherry

   Plasmid

   #99881

   Purpose

   Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Mutation

   K162M (kinase-dead)

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET30-ARH3-His-Sumo-HA

   Plasmid

   #111578

   Purpose

   Expresses ARH3 (amino acids 14-363) in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   ARH3 (ADPRS Human)
   

   Use

   Tags

   6xHis-Sumo and HA

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-13

   Promoter

   T7

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-His6-Ran(Q69L)

   Plasmid

   #171563

   Purpose

   Encodes human Ran protein

   Depositor

   Yan Zeng

   Insert

   Ran (RAN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-45b-mCherry-Cbx5

   Plasmid

   #194547

   Purpose

   Bacterial Expression of 6xHis mCherry-HP1a Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   Cbx9 (HP1a)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-MBP-CBX1-eReader

    Plasmid

    #135089

    Purpose

    Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-45b-mEGFP-HMGB1-WT

    Plasmid

    #194543

    Purpose

    Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-MBP-NLS-Geo_st

    Plasmid

    #87703

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Use

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-6His-SUMO-Nsp10

    Plasmid

    #154959

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pET-6xHis-(pos15)GFP

    Plasmid

    #89248

    Purpose

    [Edit] Expresses GFP with +15 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos15)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-6xHis-(pos14)GFP

    Plasmid

    #89252

    Purpose

    Expresses GFP with +14 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-21a(+)-AK1-His

    Plasmid

    #118977

    Purpose

    Bacterial expression plasmid for Adenylate kinase isoenzyme 1

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-mOct4 Full length(FL)

    Plasmid

    #206394

    Purpose

    Expresses Oct4 with a 6xHis tag for protein purification

    Depositor

    Ralf Jauch

    Insert

    Oct4 (Pou5f1 Mouse)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-6XHis-SUMO-SIRT2

    Plasmid

    #102622

    Purpose

    Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Tags

    6XHis-SUMO

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-37 and 357-389

    Promoter

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-45b-mCherry-NPM1

    Plasmid

    #194546

    Purpose

    Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    NPM1 (Npm1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-Sac-Abeta(MC1-42)

    Plasmid

    #127151

    Purpose

    Expresses N-terminal cysteine Abeta(1-42)

    Depositor

    James Nowick

    Article

    Zhang et al Biochemistry. 2021 Apr 20;60(15):1191-12

    Insert

    Abeta(MC1-42) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge