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pLV-Puro_CI-hCOL4A5-Nluc_S36X

Plasmid

#198108

Purpose

lentiviral expression of hCOL4A4_S36X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

S36X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc

Plasmid

#198106

Purpose

lentiviral expression of hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Promoter

EF1a

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-BTLA (PD-1 ITIM)-mGFP

Plasmid

#180818

Purpose

Expression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFP

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

BTLA (PD-1 ITIM) (BTLA Human)

Use

Lentiviral

Tags

mEGFP

Mutation

replaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…

Promoter

SFFV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-u(XXL)Cas9

Plasmid

#127387

Purpose

Expresses Cas9 at very low level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(XXL)-Cas9

Use

CRISPR

Expression

Insect

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCKIPSD-1/1

Plasmid

#91376

Purpose

Protein expression and purification of human SH3 domain construct NCKIPSD-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCKIPSD-1/1 (NCKIPSD Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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UAS-u(XS)Cas9

Plasmid

#127382

Purpose

Expresses Cas9 at very high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF-Cas9

Use

CRISPR

Expression

Insect

Availability

Academic Institutions and Nonprofits only

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pGY577

Plasmid

#166663

Purpose

Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Cre: E340A, D341A

Promoter

CMV

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGY416

Plasmid

#166660

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

GAL1

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc

Plasmid

#198113

Purpose

Express hCOL4A5 in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-FRT-GFP-FRT-u(M)Cas9

Plasmid

#127388

Purpose

Cas9 inducible by removal of a GFP flip-out cassette

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

FRT-GFP-FRT-uORF(M)Cas9

Use

CRISPR

Expression

Insect

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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