We narrowed to 5,122 results for: NOG

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pUPD2 linker-RDF (GB2892)

Plasmid

#160604

Purpose

RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha2 P35S:RDF:T35S (GB1508)

Plasmid

#160613

Purpose

TU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X-ORF24-NTD-3L_A

Plasmid

#138466

Purpose

Expresses MBP-tagged ORF24-NTD 3L_A in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

MBP

Expression

Bacterial

Mutation

Amino acids 1-201; amino acids L73-75 mutated to …

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pQLink-GST-2xCTD

Plasmid

#138470

Purpose

Expresses GST-tagged 2xCTD heptapeptide repeat in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Tags

GST

Expression

Bacterial

Mutation

2x CTD repeat

Promoter

T5

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L73A_L75A-3xFLAG

Plasmid

#138429

Purpose

Expresses full-length ORF24 L73A/L75A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L73, L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L74A_L75A-3xFLAG

Plasmid

#138430

Purpose

Expresses full-length ORF24 L74A/L75A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L74, L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 R68A-3xFLAG

Plasmid

#138431

Purpose

Expresses full-length ORF24 R68A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid R68 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 R72A-3xFLAG

Plasmid

#138432

Purpose

Expresses full-length ORF24 R72A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid R72 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L73A_L74A-3xFLAG

Plasmid

#138433

Purpose

Expresses full-length ORF24 L73A/L74A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L73, L74 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-UL87 3L_A-2xStrep

Plasmid

#138437

Purpose

Expresses full-length UL87 3L_A with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87 (UL87 HCMV (HHV-5))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids L90-L92 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-BcRF1 3L_A-2xStrep

Plasmid

#138439

Purpose

Expresses full-length BcRF1 3L_A with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids L64-L66 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 3L_A-2xStrep

Plasmid

#138440

Purpose

Expresses full-length ORF24 3L_A with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

Strep

Expression

Mammalian

Mutation

Amino acids L73-L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 202-752-3xFLAG

Plasmid

#138424

Purpose

Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 202-752 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 202-752

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 3L_A-3xFLAG

Plasmid

#138425

Purpose

Expresses full-length ORF24 3L_A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids L73-L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L73A-3xFLAG

Plasmid

#138426

Purpose

Expresses full-length ORF24 L73A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid L73 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L74A-3xFLAG

Plasmid

#138427

Purpose

Expresses full-length ORF24 L74A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid L74 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24 L75A-3xFLAG

Plasmid

#138428

Purpose

Expresses full-length ORF24 L75A with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid L75 mutated to alanine

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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563aa ELL2 pEF4B

Plasmid

#127273

Purpose

Expresses 563aa human WT ELL2

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 truncated at SphI (ELL2 Human)

Expression

Mammalian

Mutation

Contains 564 N-terminal amino acids ELL2, termina…

Promoter

pEF1a

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Sph ELL2

Plasmid

#127270

Purpose

For in vitro translation of human WT ELL2 with internal HA tag

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

WT Mets and HA tag at SphI site (8th Met)

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged mid ELL2

Plasmid

#127274

Purpose

Expresses human ELL2 with HA tag in the middle that does not disrupt function

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 with HA at XbaI (ELL2 Human)

Expression

Mammalian

Mutation

HA inserted at XbaI after Met 186

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met138ILeu ELL2

Plasmid

#127260

Purpose

For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met138 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met186ILeu ELL2

Plasmid

#127261

Purpose

For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met186 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met186 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met138ILeu, Met186ILeu ELL2

Plasmid

#127265

Purpose

For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Two Mets (138,186) to ILeu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met133ILeu ELL2

Plasmid

#127259

Purpose

For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met133 to Ileu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Met133ILeu, Met186ILeu ELL2

Plasmid

#127264

Purpose

For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Two Mets (133,186 )to ILeu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Met331Leu ELL2

Plasmid

#127262

Purpose

For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met331 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met331 to ILeu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
COOHend ELL2

Plasmid

#127255

Purpose

Expresses the C terminal segment of human ELL2 in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (C-terminal end) (ELL2 Human)

Use

Other

Expression

Mammalian

Mutation

contains only the last 389 aa

Promoter

pEF1a

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Met1ILeu ELL2

Plasmid

#127258

Purpose

For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met1 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met1 to Ileu by Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ELL2mRNA+polyA

Plasmid

#127256

Purpose

For in vitro translation of human ELL2 with 30 nt pA tail

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

shortens 3'UTR to 40 bp adds A30 tail

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge