We narrowed to 22,314 results for: CRISPR

1. pX330A-1x7

   Plasmid

   #58771

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYC1876

   Plasmid

   #158722

   Purpose

   Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   ssrA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMiniT:SEP-GluA3 TKIT donor

   Plasmid

   #169425

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA3 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Anep8_Cas9

   Plasmid

   #117169

   Purpose

   Expressed Cas9 in Aspergillus with LIC tags for easy gRNA cloning

   Depositor

   Adrian Tsang

   Article

   Song et al PLoS One. 2018 Aug 24;13(8):e0202868. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Filamentous fungi e…

   Tags

   Expression

   Mutation

   Promoter

   Pki

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLH-sgRNA1-PP7-boxB

   Plasmid

   #75393

   Purpose

   sgRNA1-PP7-boxB

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

   Insert

   sgRNA1-2XPP7-boxB
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC2_7_dCas9_HC_sgRNA

   Plasmid

   #163711

   Purpose

   High copy plasmid with dCas9 and sgRNA expression cassettes

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3 gRNA concatemer

   Plasmid

   #84880

   Purpose

   Empty concatmer vector in which 3 gRNAs can be inserted

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pJYScr

   Plasmid

   #132719

   Purpose

   Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

   Depositor

   Jan Marienhagen

   Article

   Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGTag Hygro EGFP nLAP +0

   Plasmid

   #194305

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(EGFP)
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB700 lenti gRNA EBFP2

    Plasmid

    #167905

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-dSa-VP64

    Plasmid

    #99680

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. OA-1050E (Ubiq-CasRx)

    Plasmid

    #132416

    Purpose

    Express CasRx under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    CasRx
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ubiq

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459_gRNA pAS single_hspCas9

    Plasmid

    #193311

    Purpose

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBRC1

    Plasmid

    #183064

    Purpose

    Base editing plasmid for Pseudomonas chlororaphis

    Depositor

    Xuehong Zhang

    Article

    Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

    Insert

    eSpCas9ppD10A
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMH183

    Plasmid

    #122856

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid

    #174140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas12c-sgRNA_BbsI_CAM

    Plasmid

    #183221

    Purpose

    Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

    Plasmid

    #183077

    Purpose

    Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c tracrRNA and crRNA ending with a HDV ribozyme
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL1P2_crU626

    Plasmid

    #191777

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pICSL1P7_crU626

    Plasmid

    #191782

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. bbCas9pluspAAA

    Plasmid

    #82581

    Purpose

    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

    Depositor

    Ivo Huijbers

    Article

    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCfB8792

    Plasmid

    #126910

    Purpose

    Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRTS-GBP-dCas9-mRFP

    Plasmid

    #102934

    Purpose

    Tetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

    Depositor

    Sebastian Bultmann

    Article

    Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GBP (GFP binding protein and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

24. lentiguide-puro-wt-ltr

    Plasmid

    #182635

    Purpose

    Expresses guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pICSL1P6_crU626

    Plasmid

    #191781

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pICSL1P3_crU626

    Plasmid

    #191778

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pICSL1P4_crU626

    Plasmid

    #191779

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICSL1P5_crU626

    Plasmid

    #191780

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYPQ147

    Plasmid

    #69299

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

    Plasmid

    #196710

    Purpose

    Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

    Depositor

    Bernhard Schmierer

    Article

    Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge