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pReporter_9

Plasmid

#60569

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_9

Expression

Bacterial

Promoter

BBa_J23119

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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UASGAL-Pgal

Plasmid

#60338

Purpose

Contains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoter

Depositor

Hal Alper

Publication

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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UASGAL-PCYC

Plasmid

#60335

Purpose

Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Publication

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

Plasmid

#52893

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Publication

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[2]

Tags

L-IFP-F[2]

Expression

Yeast

Promoter

GAL1

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pCAGGS-C-TEV-Twin-Strep

Plasmid

#202527

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Publication

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-41

Plasmid

#172757

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-YdLight1

Plasmid

#125705

Purpose

Expresses the yellow genetically encoded dopamine sensor YdLight1 in mammalian cells.

Depositor

Lin Tian

Publication

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Insert

YdLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-GLI2shR

Plasmid

#136691

Purpose

Inducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2 5′CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGGTAGTCATGCCAGGTTTTTG 3′)

Depositor

LuZhe Sun

Publication

Zhu et al Lab Invest. 2018 Nov;98(11):1384-1396. d

Insert

shRNA_humanGli2 (GLI2 Human)

Use

Lentiviral

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP2

Plasmid

#129500

Purpose

Expresses AausFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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MTK2_023

Plasmid

#123715

Purpose

Encodes pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-WDR5 shRNA 2

Plasmid

#59975

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pNHA7

Plasmid

#223649

Purpose

birA protein purification

Depositor

François-Xavier Campbell-Valois

Publication

Haidar-Ahmad et al mSphere. 2024 Nov 21;9(11):e0055324. doi

Insert

BioID_G118R

Tags

6His

Expression

Bacterial

Mutation

G118R

Promoter

LacI

Available since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-BBS5

Plasmid

#218714

Purpose

Expresses N-terminally EGFP-tagged BBS5 in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS5 (BBS5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS4

Plasmid

#218713

Purpose

Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS4 (BBS4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

I354T (t1110c)

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot9_S

Plasmid

#147494

Purpose

Bacterial Expression of HsNot9

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot9 (CNOT9 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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p7SK ST

Plasmid

#188714

Purpose

sgRNA plasmid encoding 7SK promoter driving an anti-safe targeting sgRNA

Depositor

David Tobin

Publication

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

Safe Targeting sgRNA

Use

CRISPR and Synthetic Biology

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-FB-E25TAG

Plasmid

#176545

Purpose

Expresses IgG affinity protein FB with a TAG stop codon at E25 position

Depositor

Han Xiao

Publication

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

IgG affinity protein

Tags

His tag

Expression

Bacterial

Mutation

change glutamic acid 25 to amber stop codon

Promoter

T5

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-FB-E25TAG-F6C

Plasmid

#176546

Purpose

Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

Depositor

Han Xiao

Publication

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

IgG affinity protein

Tags

His tag

Expression

Bacterial

Mutation

change phenylalanine 6 to cysteine; change glutam…

Promoter

T5

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF037

Plasmid

#157634

Purpose

Batis maritima MHT

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

MHT

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-bsr

Plasmid

#165878

Purpose

Basic Blasticidin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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