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psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

Plasmid

#61790

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-1 mutated

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

Plasmid

#61789

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated

Use

Luciferase

Tags

Expression

Mutation

both miR-200c MRE-2 and MRE-3 mutated

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

Plasmid

#61786

Purpose

Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Crtap 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-1 mutated

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-R5A-K6A C11orf83-V5

Plasmid

#65850

Purpose

Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6A

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Mutated UQCC3 (UQCC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

R5A-K6A

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-R5D-K6D C11orf83-V5

Plasmid

#65851

Purpose

Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5D-K6D

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Mutated UQCC3 (UQCC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

R5D-K6D

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

Plasmid

#61787

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR with miR-200c MRE-2 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-2 mutated

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Tob1-3'UTR-MRE-1 MUT

Plasmid

#61788

Purpose

Luciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Tob1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-1 mutated

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Zfp36-3'UTR-MRE-1 MUT

Plasmid

#61794

Purpose

Luciferase reporter containing the 3'UTR of mouse Zfp36 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zfp36 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-1 mutated

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Snail1-3'UTR-MRE-1 MUT

Plasmid

#61795

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-1 mutated

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR-MRE-1 MUT

Plasmid

#61797

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Tags

Expression

Mutation

miR-200c MRE-1 mutated

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
STIM1-EGFP

Plasmid

#210370

Purpose

Expresses STIM1 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

STIM1 (STIM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-V0A

Plasmid

#210347

Purpose

Expresses ATP6V0A in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

V-ATPase V0A (ATP6V0A1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

Plasmid

#113865

Purpose

Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

Plasmid

#199371

Purpose

Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-ALFA nanobody

Use

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Plxna1-Fc-His

Plasmid

#72122

Purpose

Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna1 (Plxna1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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Plxna2-Fc-His

Plasmid

#72123

Purpose

Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna2 (Plxna2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Plxna3-Fc-His

Plasmid

#72124

Purpose

Expresses the extracellular region of the PlexinA3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna3 (Plxna3 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSPObooster

Plasmid

#216160

Purpose

Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

Depositor

Grant Brown

Article

Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

Inserts

MKT1(30G) (MKT1 Budding Yeast)
RME1(ins-308A) (RME1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

MKT1 gene containing an aspartic acid to glycine …

Promoter

endogenous promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR-mcBEST

Plasmid

#209416

Purpose

Plasmid for multiplexed cytosine base editing in streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

Inserts

Codon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

base editing cassette: PtipA ; sgRNAs: PkasO* an…

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAcUW51-gEgIL

Plasmid

#11622

Depositor

Pamela Bjorkman

Article

Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

Insert

HSV-1 glycoprotein E and HSV-1 glycoprotein I

Use

Tags

His

Expression

Insect

Mutation

Dicistronic vector containing HSV-1 gE (KOS strai…

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only