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pBD34

Plasmid

#245769

Purpose

Expresses tiam-1::mGFP in cholinergic motor neurons

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

tiam-1::mGFP

Tags

mGFP

Expression

Worm

Promoter

unc-17b

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBD31

Plasmid

#245768

Purpose

Expresses rap-1::mGFP in cholinergic motor neurons

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rap-1::mGFP

Tags

mGFP

Expression

Worm

Promoter

unc-17b

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP2-VAV1-E201A

Plasmid

#242838

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

E201A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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MBP-GFP-RGG

Plasmid

#242453

Purpose

Expresses the MBP-GFP-RGG fusion protein.

Depositor

Benjamin Schuster

Article

Kelley et al Proc Natl Acad Sci U S A. 2021 Dec 21;11

Insert

MBP-GFP-RGG

Tags

His and MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GFP-RGG

Plasmid

#242454

Purpose

Expresses the GST-GFP-RGG fusion protein.

Depositor

Benjamin Schuster

Article

Kelley et al Proc Natl Acad Sci U S A. 2021 Dec 21;11

Insert

GST-GFP-RGG

Tags

GST and His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-WASP-3D

Plasmid

#242836

Purpose

Encodes human WASP and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

WASP (WAS Human)

Tags

EGFP

Expression

Mammalian

Mutation

F244D, H246D, H249D

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-hPLEKHA7-Myc

Plasmid

#236538

Purpose

For expression of PLEKHA7 (human) in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al Tissue Barriers. 2014 Jan 1;2(1):e28755.

Insert

PLEKHA7 (PLEKHA7 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pJPC060

Plasmid

#234535

Purpose

TRV2 expressing FLPe

Depositor

Daniel Voytas

Article

Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

Insert

FLPe recombinase

Use

Recombinase

Expression

Plant

Promoter

CaMV 35S

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-mCherry-SopF

Plasmid

#229745

Purpose

Expresses mCherry-tagged SopF in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

mCherry-SopF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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MbCas12a_K487_GPG_GR2

Plasmid

#231577

Purpose

Encodes a cortisol inducible variant of MbCas12a

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

MbCas12a with a GR2 insertion behind K487

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pB4GWnY-MpPHOT

Plasmid

#228472

Purpose

Vector for the expression of MpPHOT fused to the N-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpPHOT

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB605-MpRTN1

Plasmid

#228475

Purpose

Binary vector for the expression of MpRTN1 fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpRTN1

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pB4GWnY-MpRTN1

Plasmid

#228477

Purpose

Vector for the expression of MpRTN1 fused to the N-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpRTN1

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pB4GWcY-MpRTN1

Plasmid

#228478

Purpose

Vector for the expression of MpRTN1 fused to the C-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpRTN1

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pB4GWcY-MpPHOT

Plasmid

#228473

Purpose

Vector for the expression of MpPHOT fused to the C-terminal half of YFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpPHOT

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDifferentiation

Plasmid

#228393

Purpose

intended to be co-transformed along with pDawn-Ag43 to generate differentiation strain (DS-mRuby2) for multipattern biofilm lithography

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Nat Commun. 2024 Nov 1;15(1):9443. doi:

Insert

pLambda-luxR+LVAtag__pLux-mRuby2

Use

Synthetic Biology

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_λHA-GFPopt

Plasmid

#210575

Purpose

Expresses lambdaHA-optimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-GFP optimal

Tags

lambda HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_λHA-GFPnonopt

Plasmid

#210576

Purpose

Expresses lambdaHA-nonoptimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-GFP nonoptimal

Tags

lambda HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm2.2

Plasmid

#210279

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm2.5

Plasmid

#210282

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm3.1

Plasmid

#210283

Purpose

Empty backbone with chloramphenicol resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm4.1

Plasmid

#210284

Purpose

Empty backbone with chloramphenicol resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm5.1

Plasmid

#210285

Purpose

Empty backbone with trimethoprim resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-7

Plasmid

#220468

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Tags

3xHA

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-7.M4

Plasmid

#220469

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Tags

3xHA

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-5

Plasmid

#221491

Purpose

NKX2-5 lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_mNeonGreen

Plasmid

#213838

Purpose

Facilitates genomic integration of the control reporter without zinc finger binding sites.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

Plasmid

#208628

Purpose

for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag

Depositor

Quanfu Ma

Insert

human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)

Tags

6xHis, Twin Strep, and mEGFP(A206K)

Expression

Bacterial

Mutation

only aa 1~41, other residues not included

Promoter

T7

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pUdO4c

Plasmid

#210233

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge