We narrowed to 16,109 results for: form

1. Neo1.h-AP-His

   Plasmid

   #71970

   Purpose

   Expresses the extracellular region of the Neogenin 1, isoform h protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Neo1.h (Neo1 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108837

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FKBP

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA

   Plasmid

   #108840

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108841

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   PYL

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108847

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   pMag

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA

   Plasmid

   #108836

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFUW-TetO-Txnip

   Plasmid

   #178437

   Purpose

   doxycycline-inducible expression of Mouse Txnip in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   Txnip (Txnip Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA

   Plasmid

   #108842

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   GAI,NLS

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPBpuro-mNG-eDHFR(69K6)-p85iSH2

   Plasmid

   #172103

   Purpose

   Mammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-p85iSH2 (Pik3r2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBV-Luc/Frag-F

    Plasmid

    #14053

    Depositor

    Joan Massague

    Article

    Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

    Insert

    c-myc promoter (-109/-3) (MYC Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    -109/-3 (relative to the P2 transcription initiat…

    Available Since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

11. KZ001: pMVP (L3-L2) P2A-eYFP + polyA

    Plasmid

    #121770

    Purpose

    pMVP L3-L2 entry plasmid, contains eYFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-eYFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. binder/tag-WT-FAK-insertionA

    Plasmid

    #190439

    Purpose

    The tag is SsrA (AANDENY). Tag is inserted after R35 of FAK.

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    FAK (Ptk2 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SsrA inserted after aa 35

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

    Plasmid

    #121834

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-Cas9(3.7) (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-IRES-tdTomato-FlagAkt2W80A

    Plasmid

    #64834

    Purpose

    cDNA of Akt2 with point mutation from Trp to Ala at position 80. This mutation confers resistance to MK-2206 drug.

    Depositor

    Eva Gonzalez

    Article

    Kajno et al Biochem J. 2015 Apr 9.

    Insert

    Akt2 (Akt2 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Point mutation Trp 80 to Ala

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

15. LA601: pMVP (L3-L2) P2A-Puro + WPRE

    Plasmid

    #121785

    Purpose

    pMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectors

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Puro + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cntn4.2-Fc-His

    Plasmid

    #72068

    Purpose

    Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Cntn4.2 (Cntn4 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSECB sgMTHFD2L_1

    Plasmid

    #106306

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2L (MTHFD2L Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSECB sgMTHFD2L_2

    Plasmid

    #106307

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2L (MTHFD2L Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CKAP2 pHAT

    Plasmid

    #175946

    Purpose

    Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

    Depositor

    Susanne Bechstedt

    Article

    McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

    Insert

    CKAP2 (Ckap2 Mouse)
    

    Tags

    6-His and StrepTagII

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJ207 Delta-cpc-KanR

    Plasmid

    #51468

    Purpose

    Deletes the cpc operon in Synechocystis and confers kanamycin resistance

    Depositor

    Anastasios Melis

    Article

    Kirst et al Biochim Biophys Acta. 2014 Oct;1837(10):

    Insert

    Replacing the cpc operon with kanamycin resistance
    

    Use

    Synthetic Biology

    Promoter

    Synechocystis endogenous cpc-operon promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-Q842gp120-8his

    Plasmid

    #100932

    Purpose

    Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pnEA-vH_His-TEV-DSep1

    Plasmid

    #174493

    Purpose

    bacterial expression of Drosophila Sep1

    Depositor

    Manos Mavrakis

    Article

    Mavrakis et al Nat Cell Biol. 2014 Apr;16(4):322-34. do

    Insert

    Sep1 (Sep1 Fly)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. KZ501: pMVP (L3-L2) P2A-Puro + polyA

    Plasmid

    #121780

    Purpose

    pMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-Puro + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. XE157 GFP-XDpr-delta PDZB-CS2+

    Plasmid

    #16765

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    XDpr-delta PDZB (dact1-b Frog)
    

    Use

    Xenopus expression

    Tags

    gfp

    Mutation

    XDpr is truncated to delete the last four amino a…

    Available Since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

25. KY901: pMVP (L3-L2) P2A-mCherry + polyA

    Plasmid

    #121766

    Purpose

    pMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-mCherry + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR223 GABARAP G116

    Plasmid

    #123082

    Purpose

    Gateway entry clone encoding human GABARAP G116

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMRX-IPU-GFP-NEK9W967A

    Plasmid

    #168271

    Purpose

    Expresses LIR (LC3-interacting region) mutant NEK9

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    NEK9 (NEK9 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 979 to alanine

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 8200 Bicistronic_GFP_ires_hygro

    Plasmid

    #64375

    Purpose

    This a retroviral expression plasmid expressing GFP along with hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    EGFP

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSB165 - pL1R3_pMAS::rLUC-I::tMAS

    Plasmid

    #123198

    Purpose

    binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    pMAS::rLUC-I::tMAS
    

    Use

    Luciferase

    Expression

    Plant

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRS306Sup35R1

    Plasmid

    #17715

    Depositor

    Susan Lindquist

    Article

    Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

    Insert

    pRS306Sup35R1 (SUP35 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    An pRS306 based integrating plasmid designed for …

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. phumanCas9 (GB0575)

    Plasmid

    #68206

    Purpose

    Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMEH60 Lamp2b MS2 HA

    Plasmid

    #168209

    Purpose

    Mammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    HA and MS2 dimer

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFL His-SDS22

    Plasmid

    #113512

    Purpose

    Expression of His-SDS22 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    SDS22 (PPP1R7 Human)
    

    Tags

    6*His

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. KN302: pMVP/SB/eCFP-DEST

    Plasmid

    #121863

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. KK901: pMVP/SB/Zeo-DEST

    Plasmid

    #121868

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

    Plasmid

    #121821

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. K339CLM G234A/215/347

    Plasmid

    #24449

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, G234A/E215C/E…

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HD201: pMVP (L3-L2) IRES-eGFP + polyA

    Plasmid

    #121747

    Purpose

    pMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    IRES2-eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. KA501: pMVP (L3-L2) pA; CMV::TETa-pA

    Plasmid

    #121801

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + CMV::TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LA001: pMVP (L3-L2) P2A-mCherry + WPRE

    Plasmid

    #121774

    Purpose

    pMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-mCherry + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSG5-H-Ras_V12

    Plasmid

    #39510

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 (HRAS Human)
    

    Use

    Eukaryotic

    Mutation

    G12V

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

42. LA201: pMVP (L3-L2) P2A-eCFP + WPRE

    Plasmid

    #121776

    Purpose

    pMVP L3-L2 entry plasmid, contains eCFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    P2A-eCFP + WPRE
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJM149

    Plasmid

    #134168

    Purpose

    Retroviral plasmid expressing GFP - Nde1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    Nde1_SSSC E118A R129A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SSSC E118A R129A, E. coli codon optimized, CRISPR…

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pJM147

    Plasmid

    #134167

    Purpose

    Retroviral plasmid expressing GFP - Nde1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    Nde1_SSSC E47A E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SSSC E47A, E. coli codon optimized, CRISPR/Cas9 h…

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA

    Plasmid

    #108838

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    PYL,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108839

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ABI

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA

    Plasmid

    #108834

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FKBP,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108835

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pTNos:BASTA:PNos (GB0023)

    Plasmid

    #68211

    Purpose

    Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    bar gene
    

    Use

    Unspecified

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    PNos

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. Ntn5.a-AP-His

    Plasmid

    #71981

    Purpose

    Expresses the entire Netrin 5, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn5.a (Ntn5 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge