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pBa.PEX 1-42-tdTM-FKBP

Plasmid

#224415

Purpose

Mammalian expression of Pex3

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Pex3 (Pex3 Mouse)

Use

Tags

tdTom-FKBP

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hNKD2

Plasmid

#174329

Purpose

encodes human NKD2 cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

MycHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Tmtc4

Plasmid

#45869

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

TMTC4 in situ probe (Tmtc4 Mouse)

Use

In situ

Tags

Expression

Mutation

nt 1220-1798 on BC031368

Promoter

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Del2 N-term GFP

Plasmid

#36133

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains CypB AA 38-210

Promoter

CMV

Available since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Nucl2 N-term GFP

Plasmid

#36125

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains CypB AA 34-211

Promoter

CMV

Available since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Del3 N-term GFP

Plasmid

#36135

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 39-210 of cyclophilin B

Promoter

CMV

Available since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
pSiP3

Plasmid

#21957

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

Pten (Pten Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

Plasmid

#102796

Purpose

Retrovirus for delivery of one sgRNA (empty, bbsI)

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

U6 sgRNA

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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L13-Arl13bGFP

Plasmid

#40879

Purpose

3rd generation transfer vector

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST47-Arl13b12

Plasmid

#40872

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

no stop codon

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[control]

Plasmid

#75438

Purpose

control shRNA vector

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

GFP

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Cep83

Plasmid

#128874

Purpose

For mammalian expression of mouse Cep83

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Cep83 (Ccdc41 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano15/pBig

Plasmid

#51962

Purpose

Expression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in Dictyostelium

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-mTagBFP2-Sec61B

Plasmid

#228136

Purpose

Expresses mTagBFP2-Sec61B in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Sec61B (SEC61B Human)

Use

Retroviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-PEX3-EGFP

Plasmid

#228142

Purpose

Expresses PEX3-GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

PEX3 (PEX3 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-SNAP-Rab7

Plasmid

#228141

Purpose

Expresses SNAP-Rab7 in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Rab7 (Rab7 Mouse)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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hsp70:Arl13b-mKate2

Plasmid

#105964

Purpose

heat shock inducible transgenesis, primary cillium marker

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

mKate2

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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Yellow Cameleon-Nano140/pcDNA3

Plasmid

#51966

Purpose

mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

Depositor

Takeharu Nagai

Article

Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

Insert

Yellow Cameleon-Nano140

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 rictor

Plasmid

#21341

Depositor

David Sabatini

Article

Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

Insert

rictor shRNA 1 (Rictor Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nrxn1beta AS4(-)-Fc

Plasmid

#59313

Purpose

Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

Depositor

Peter Scheiffele, Tito Serafini

Article

Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Insert

Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)

Use

Tags

Fc (from human IgG)

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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