We narrowed to 386 results for: SaCas9

1. SpeCas9-HF

   Plasmid

   #192141

   Purpose

   Expresses highly specific SpeCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   SpeCas9-HF
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   SpeCas9-HF (R247A, N415A, S421A)

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

   Plasmid

   #113684

   Purpose

   EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   N/A
   

   Use

   AAV

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

   Plasmid

   #109318

   Purpose

   Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   SaCas9 RuvC gRNA
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Sha2Cas9-HF

   Plasmid

   #192142

   Purpose

   Expresses highly specific Sha2Cas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   Sha2Cas9-HF
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Sha2Cas9-HF (R247A and N415A)

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 1029-AIDx-CP

   Plasmid

   #135341

   Purpose

   Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   CP 1029 nSpyCas9-hAIDx
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. S2 1058 ABEMax

   Plasmid

   #135356

   Purpose

   Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-ABEMax aa 1058
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. S2 1058 V82G ABEMax

   Plasmid

   #135361

   Purpose

   Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1058 nSpyCas9 V82G ABEMax
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AIDx-ID-202

   Plasmid

   #135344

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-202
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AIDx-ID-208

   Plasmid

   #135345

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-208
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AIDx-ID-468

    Plasmid

    #135346

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-468
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 1010-AIDx-CP

    Plasmid

    #135340

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1010 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1010 nSpyCas9 -hAIDx
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 1058-AIDx-CP

    Plasmid

    #135342

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1058 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1058 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

    Plasmid

    #89991

    Purpose

    Donor template for generation of SOX2-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
    

    Use

    CRISPR

    Promoter

    none

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PDG459 V3

    Plasmid

    #226958

    Purpose

    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PDG458 V3

    Plasmid

    #226959

    Purpose

    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PX458 V3

    Plasmid

    #226957

    Purpose

    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ABEmax-Puro V3

    Plasmid

    #226956

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Pdg459-eSp(1.1) V3

    Plasmid

    #226965

    Purpose

    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPapi

    Plasmid

    #96921

    Purpose

    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZac2.1 U6-SaAi9L-U6-SaAi9R

    Plasmid

    #78604

    Purpose

    AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    gRNAs for SaCas9 targeting Ai9 locus
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only