We narrowed to 6,904 results for: crispr cas9 plasmids

1. NAX_CAGGS_CAS9_m4_Hygro

   Plasmid

   #167867

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_m4_EBFP2

   Plasmid

   #167865

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-dCas9_puroR

   Plasmid

   #219825

   Purpose

   CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

   Depositor

   Philipp Maass

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   CMV

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGM4723:Ble:Cas9

   Plasmid

   #112208

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

   Depositor

   Thomas Mock

   Inserts

   Cas9
   SheBle
   

   Use

   CRISPR; Diatom, phaeodactylum

   Tags

   Phaeodactylum fcp promoter and terminator and YFP

   Expression

   Mutation

   Phaeodactylum Fcp promoter

   Promoter

   Phaeodactylum Fcp

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-MP-84-spCas9-U6-gRNA

   Plasmid

   #206935

   Purpose

   Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

   Depositor

   Markus Grompe

   Insert

   spCas9
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   MP-84

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRE3G-dCas9-VP64-MS2-VP64-ZsG

   Plasmid

   #192669

   Purpose

   Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   TRE3G

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

   Plasmid

   #117134

   Purpose

   All-in-one expression vector for Cas9 and gRNA against CD44

   Depositor

   Kosuke Yusa

   Insert

   gCD44 v2 (Cd44 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   human U6 promoter, human EF1a promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-Cas9-Exo-Blast-BFP

   Plasmid

   #196721

   Purpose

   Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-Exo1-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. mPLD3-sgRNA-Cas9-mcherry

    Plasmid

    #199700

    Purpose

    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVTR(T7A)-Cas9-T2A-mClover3

    Plasmid

    #172293

    Purpose

    In vitro transcription of Cas9-T2A-mClover3 synthetic construct

    Depositor

    Patrik Verstreken

    Insert

    Cas9-T2A-mClover3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZ8-P_dCas9

    Plasmid

    #74063

    Purpose

    pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    dcas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Propionate inducible promoter (prp)

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9-EcRT-GAL-Hyg

    Plasmid

    #232091

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas9-EcRT-GAL-HIS3

    Plasmid

    #232093

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas9-EcRT-GAL-Nat

    Plasmid

    #232090

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9-EcRT-GAL-Kan

    Plasmid

    #232092

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid

    #196720

    Purpose

    Tet-inducible expression of Cas9-T2A-TagBFP

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    TRE, PGK

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid

    #196722

    Purpose

    Tet-inducible expression of Cas9-T2A-miRFP670nano

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    TRE, PGK

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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