We narrowed to 4,850 results for: U6...

1. LentiCRISPR-sgC17orf89-2

   Plasmid

   #86135

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   sgRNA targeting C17orf89 (NDUFAF8 )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCm7RNA6A

   Plasmid

   #84367

   Purpose

   Cas9-gRNA plasmid for mouse Cbfa2t2 m7 mutant knockin

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   Cbfa2t2 m7-gRNA6
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

3. mCBFKOgRNA5

   Plasmid

   #84366

   Purpose

   Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   Cbfa2t2-gRNA5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

4. mCBFKOgRNA2

   Plasmid

   #84365

   Purpose

   Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   Cbfa2t2-gRNA2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

5. TU#1805_CRISPR_unc-73_exon2

   Plasmid

   #82359

   Purpose

   to create unc-73B null allele

   Depositor

   Martin Chalfie

   Article

   Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

   Insert

   Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJZC103

   Plasmid

   #62340

   Purpose

   sgRNA for mammalian cells with mCherry marker

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Insert

   sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. RFN_EGFP_84

   Plasmid

   #60076

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. RFN_EGFP_83

   Plasmid

   #60075

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. RFN_EGFP_82

   Plasmid

   #60074

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. RFN_EGFP_80

    Plasmid

    #60072

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSicoR-CENPBi2

    Plasmid

    #59619

    Purpose

    Expression of shRNA against human CENPB

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    CENPB (CENPB Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pSicoR-ADAM29i2

    Plasmid

    #59607

    Purpose

    Expression of shRNA against human ADAM29

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROP-seq-opti

    Plasmid

    #106280

    Purpose

    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    EF1a-Puro-WPRE-hU6-gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-PITCh-H3C2

    Plasmid

    #207780

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgRNA(MS2) cloning backbone

    Plasmid

    #61424

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDGE334

    Plasmid

    #153243

    Purpose

    M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXAT2

    Plasmid

    #80494

    Purpose

    AAVS1 sgRNA expression vector

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    AAVS1 sgRNA-T2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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