...slightly lower expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA...shRNA From the human U6 small nuclear promoter Constitutive  Murine U6 is also used, but may be less efficient...

...the years on their own or through Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9...cloned to create a chimeric guide RNA. Find pX330-U6-Chimeric_BB-CBh-hSpCas9. pSpCas9(BB)-2A-Puro (PX459...chimeric RNA element with customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a ...

...target sequence downstream of the 7SK, human U6, mouse U6, or human H1 promoters. If you express fewer...modification of pX330, contains humanized wtCas9 and two U6 promoters. To use this plasmid, you simply order ...and up to three gRNAs. Entry vectors containing the U6 promoter and the gRNA scaffold are provided with ... cloning. gRNAs are expressed from two Drosophila U6 promoters. Cas9 must be supplied on a separate plasmid...

...target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary to ...to the start of my target sequence? A2: The human U6 promoter prefers a 'G' at the transcription start...

... first site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the...containing a direct fusion of the desired gRNA to the U6 promoter, and a second plasmid containing the homology...

... upstream of the Cbh promoter, this plasmid has a U6 promoter followed by BbsI restriction sites into ...

...why pC016 - LwCas13a guide expression backbone with U6 promoter has already earned a blue flame. RNA Editing...

...strategy, the need for lengthy promoters, such as U6 or EF1α promoters, has limited the number of gRNAs...

...Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA...

...Cas9 expression can be constitutive or inducible. U6 promoter is typically used for gRNA. May contain ...

...expresses S. pyogenes Cas9 from the PGK promoter) and U6 promoter-driven guide RNA constructs, pAdSh.U6.gRNAS1...

...Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 74009 Zebrafish U6 yes, cut S. pyogenes EGFP Del Bene pUAS:Cas9T2ACre;U6:sgRNA1... Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian U6 yes, cut N. meningitidis...LEU2 Lu pDestTol2CG2-U6:gRNA 63156 Zebrafish BseRI none S. pyogenes Zon pDestTol2pA2-U6:gRNA 63157 Zebrafish...pSmart-Nm-sgRNA-BbsI 49157 Mammalian U6 none N. meningitidis Thomson pXCas9H840A 60900 Mammalian U6 yes, nick S. pyogenes...Mammalian/Lentiviral U6 none S. pyogenes Pederson pLH-nmsgRNA1.1 64115 Mammalian/Lentiviral U6 none N. meningitidis.../Lentiviral U6 none N. meningitidis Pederson pLH-stsgRNA2.1 64117 Mammalian/Lentiviral U6 none N. meningitidis...Lentiviral U6 none N. meningitidis Pederson pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6 64220 Mammalian U6 yes,...

...46914 pU6-sgGFP-NT1 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1...46917 pU6-sgCXCR4-2 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous...46915 pU6-sgGAL4-1 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS...46916 pU6-sgGAL4-4 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS...46918 pU6-sgCD71-2 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous...second plasmid ( pgRNA_humanized ) contains a murine U6 promoter controlled sgRNA cassette, wherein the GN19...

...are described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...PX601; CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600...promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; for sgRNA cloning; has GFP-KASH ...binding globulin (TBG, PX602 [#61593] ) promoter, and a U6-driven single guide RNA. The vector can be digested...acceptor: The PX552 plasmid (#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor)... directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This ...is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This...

...uniquely transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence by flow cytometry...

...53062 pU6-gRNA wheat U6 BbsI none S. pyogenes Gao 59188 pBlu/gRNA Arabidopsis U6 BbsI none S. pyogenes...pyogenes Hyg Chen 78098 pHAtC U6 AarI yes, cut S. pyogenes Hygro Kim 78097 pBAtC U6 AarI yes, cut S. pyogenes...Basta Kim 71286 pHEE401 U6-26p BsaI yes, cut S. pyogenes Hygro Chen 71287 pHEE401E U6-26p BsaI yes, cut S....aU6 BsaI none S. pyogenes Kamoun 52255 pUC119-gRNA U6 PCR template none S. pyogenes Sheen 51295 pRGEB31...pyogenes Hygro Chen 78932 pHDE-35S-Cas9-mCherry-UBQ U6-26 PmeI yes, cut S. pyogenes Hygro, mCherry Zhao ...

...Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon 149546 pCFD5-NS Drosophila Drosophila U6:3 pegRNA...Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI + PstI...Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 176901 U6-pe RNA-H1-nick sgRNA-mCherry Mammalian...

... 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748 pLKO.3G 3rd U6-driven...Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. ...Trono 11795 pLL3.7 3rd Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included ...silencing were added; Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included ...

...cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30...of the available sgRNA-cargo constructs utilize a U6 promoter and contain the RNA cargo cassette inserted...

...regulatory sequences of an RNA polymerase III transcribed U6 snRNA. In both vectors, the target site sequence ... 3xP3-DsRed. (Also known as pHD-DsRed-att). 51026 U6-BbsI-crRNA : Generates crRNA for use in combination...