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  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 74009 Zebrafish U6 yes, cut S. pyogenes EGFP Del Bene pUAS:Cas9T2ACre;U6:sgRNA1... Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868 Mammalian U6 yes, cut N. meningitidis...LEU2 Lu pDestTol2CG2-U6:gRNA 63156 Zebrafish BseRI none S. pyogenes Zon pDestTol2pA2-U6:gRNA 63157 Zebrafish...pSmart-Nm-sgRNA-BbsI 49157 Mammalian U6 none N. meningitidis Thomson pXCas9H840A 60900 Mammalian U6 yes, nick S. pyogenes...Mammalian/Lentiviral U6 none S. pyogenes Pederson pLH-nmsgRNA1.1 64115 Mammalian/Lentiviral U6 none N. meningitidis.../Lentiviral U6 none N. meningitidis Pederson pLH-stsgRNA2.1 64117 Mammalian/Lentiviral U6 none N. meningitidis...Lentiviral U6 none N. meningitidis Pederson pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6 64220 Mammalian U6 yes,...

  2. Qi Lab CRISPR Page

    Type
    Collection
    ...46914 pU6-sgGFP-NT1 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1...46917 pU6-sgCXCR4-2 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous...46915 pU6-sgGAL4-1 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS...46916 pU6-sgGAL4-4 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS...46918 pU6-sgCD71-2 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous...second plasmid ( pgRNA_humanized ) contains a murine U6 promoter controlled sgRNA cassette, wherein the GN19...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...are described here. #60224 - AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR...PX601; CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592 : PX600...promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; for sgRNA cloning; has GFP-KASH ...binding globulin (TBG, PX602 [#61593] ) promoter, and a U6-driven single guide RNA. The vector can be digested...acceptor: The PX552 plasmid (#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor)... directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This ...is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This...

  4. CRISPR Plasmids - Plants

    Type
    Collection
    ...53062 pU6-gRNA wheat U6 BbsI none S. pyogenes Gao 59188 pBlu/gRNA Arabidopsis U6 BbsI none S. pyogenes...pyogenes Hyg Chen 78098 pHAtC U6 AarI yes, cut S. pyogenes Hygro Kim 78097 pBAtC U6 AarI yes, cut S. pyogenes...Basta Kim 71286 pHEE401 U6-26p BsaI yes, cut S. pyogenes Hygro Chen 71287 pHEE401E U6-26p BsaI yes, cut S....aU6 BsaI none S. pyogenes Kamoun 52255 pUC119-gRNA U6 PCR template none S. pyogenes Sheen 51295 pRGEB31...pyogenes Hygro Chen 78932 pHDE-35S-Cas9-mCherry-UBQ U6-26 PmeI yes, cut S. pyogenes Hygro, mCherry Zhao ...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Drosophila U6:3 pegRNA BbsI No Vermilion Norbert Perrimon 149546 pCFD5-NS Drosophila Drosophila U6:3 pegRNA...Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI + PstI...Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 176901 U6-pe RNA-H1-nick sgRNA-mCherry Mammalian...

  6. Lentivirus Plasmids

    Type
    Collection
    ... 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748 pLKO.3G 3rd U6-driven...Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. ...Trono 11795 pLL3.7 3rd Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included ...silencing were added; Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included ...

  7. FlyCRISPR

    Type
    Collection
    ...regulatory sequences of an RNA polymerase III transcribed U6 snRNA. In both vectors, the target site sequence ... 3xP3-DsRed. (Also known as pHD-DsRed-att). 51026 U6-BbsI-crRNA : Generates crRNA for use in combination...

  8. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...reporters via an H2B fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids...shuttle plasmids to insert a second guide within a U6-guide cassette. This process is compatible with all...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Yildiz 159787 pGL3-U6-pegRNA_KCNA1-EGFP KCNA1 U6 Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1-mcherry...190900 pAAV2ss-U6-sgHTT1-7sk-sgCas9 HTT U6, 7sk Huntington's Nicole Deglon 190901 pAAVss-U6-sgHTT51-7sk-...7sk-Cas9 HTT U6, 7sk Huntington's Nicole Deglon 190902 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1 HTT GFP U6, EFS Huntington's...Deglon 190903 pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9 HTT GFP U6, EFS Huntington's Nicole Deglon 191487...78622 pX335_HR_Prnp_3 PRNP U6 Dementia Walker Jackson 78623 pX459_HR_Prnp_3 PRNP U6 Dementia Walker Jackson...MS2)_Prnp_SAM1 PRNP U6 Dementia Walker Jackson 78625 sgRNA(MS2)_Prnp_SAM2 PRNP U6 Dementia Walker Jackson...SpCas9 TARDBP U6 ALS Rajat Rohatgi 107858 pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9 TARDBP U6 ALS Rajat Rohatgi...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3 BbsI Injection ...Virmilion none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 ...

  12. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...47549 pDD162 (Peft-3::Cas9 + Empty sgRNA) R07E5.16 U6 yes, cut Goldstein 42250 DR274 T7 BsaI In vitro transcription...

  13. Retrovirus Plasmids

    Type
    Collection
    ...and Neo resistance Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression; also expresses...

  14. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...target sequence into a backbone that uses the human U6 promoter to drive expression, is it necessary to ...nucleotide to the start of my target sequence? The human U6 promoter prefers a 'G' at the transcription start...

  15. Cre-lox system

    Type
    Collection
    ... Nanog locus mNanog Mammalian Hanna 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre... and sgRNA expression EFS AAV Zhang 60229 AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR Cre and sgRNA... sgRNA coexpression Cbh AAV Zhang 60231 AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-...lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre Cre Chicken cardiac troponin T AAV Pu...

  16. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...shRNA against mouse TNF-alpha mRNA from the mouse U6 promoter pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro 73022 ...

  17. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...of the 20-mer is not G. sgRNA expression from the U6 promoter of the pX330 vector is enhanced by the inclusion... for each sample and sequence each colony using a U6 promoter forward primer: CGTAACTTGAAAGTATTTCGATTTCTTGGC...

  18. Mammalian RNAi Tools

    Type
    Collection
    ...Sabatini 11795 pLL3.7 Expresses shRNA under the mouse U6 promoter; A CMV-EGFP reporter cassette is included...

  19. Plan Your Experiment

    Type
    Collection
    ...constitutive (CMV, EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA May contain reporter...

  20. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...
Showing: 1 - 20 of 20 results