We narrowed to 13,040 results for: Mpl

1. pmiRGLO-SEMA5B-202

   Plasmid

   #117362

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR SEMA5B-202
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmiRGLO-SEMA5B-211

   Plasmid

   #117363

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR SEMA5B-211
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmiRGLO-RPLP1-201

   Plasmid

   #117361

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR RPLP1-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmiRGLO-ASIC1

   Plasmid

   #117339

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR ASIC1
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmiRGLO-ARF1-214

   Plasmid

   #117337

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR ARF1-214
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pmiRGLO-LEMD2-201

   Plasmid

   #117349

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR LEMD2-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmiRGLO-PPM1F-201

   Plasmid

   #117334

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR PPM1F-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmiRGLO-GLIS2-201

   Plasmid

   #117331

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR GLIS2-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmiRGLO-CERS2-201

   Plasmid

   #117330

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR CERS2-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19_T790M_28bp

    Plasmid

    #114721

    Purpose

    28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    c.2369C>T (Substitution, position 2369, C➞T); …

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-6P-1 p37deltaSEP

    Plasmid

    #113501

    Purpose

    Bacterial expression of GST-tagged p37 lacking the SEP domain

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 140-206

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-6P-1 p37delta2-81

    Plasmid

    #113502

    Purpose

    Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 2-81

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFL His-I3

    Plasmid

    #113513

    Purpose

    Expression of His-I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19_4xT790M_28bp

    Plasmid

    #114719

    Purpose

    4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard

    Depositor

    Nils Walter

    Article

    Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    c.2369C>T (Substitution, position 2369, C➞T); …

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a_6HT-TBP6.7(R47K)

    Plasmid

    #112723

    Purpose

    Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47K)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47K

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a_6HT-TBP6.7(delta C-term)

    Plasmid

    #112731

    Purpose

    This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(delta C-term)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    delta C-term

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. [pSK480] pLJC5 Flag-Depdc5(A)

    Plasmid

    #109338

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant A

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    DIYGD(185-189)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. [pSK481] pLJC5 Flag-Depdc5(B)

    Plasmid

    #109339

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant B

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    EQPLH(371-375)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. [pSK479] pLJC5 Flag-Depdc5(C)

    Plasmid

    #109340

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant C

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    FTPRI(419-423)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCR3-vsv-dCEN-Incenp

    Plasmid

    #108479

    Purpose

    expression of vsv-dCEN-INCENP

    Depositor

    Susanne Lens

    Article

    Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

    Insert

    dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    CEN box removed

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSG5-chCAR

    Plasmid

    #87231

    Purpose

    Mammalian expression of chicken CAR

    Depositor

    Fritz Rathjen

    Article

    Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

    Insert

    CXADR (CXADR Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJOG252

    Plasmid

    #80584

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJOG253

    Plasmid

    #80585

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCIG1_UL136_26kDa_Myc

    Plasmid

    #74955

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)

    Expression

    Mammalian

    Mutation

    delta (1-62) M78A M80A M100A M128A

    Promoter

    CMV, Lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCIG1_UL136_33kda_Myc

    Plasmid

    #74957

    Purpose

    Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Insert

    UL136 (UL136 Human Cytomegalovirus strain TB40/E)
    

    Use

    Lentiviral

    Tags

    Myc tag (EQKLISEEDL)

    Expression

    Mammalian

    Mutation

    M63A M78A M80A M100A M128A

    Promoter

    CMV, Lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5 FRT TO myc Separase delta 1278-1291

    Plasmid

    #59830

    Purpose

    Allows the integration of myc Separase delta 1278-1291 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 1278-1291

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcdna3 T7 Jip1b(471-707)(C478S and C699T)

    Plasmid

    #52132

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-470 and changed C478S and C6…

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pcdna3 T7 Jip1b(282-470)

    Plasmid

    #52125

    Purpose

    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

    Depositor

    Roger Davis

    Article

    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

    Insert

    JNK interacting protein-1b (JIP-1b)(282-470) (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-281 and 471-707

    Promoter

    Available since

    April 18, 2014

    Availability

    Academic Institutions and Nonprofits only

33. LV-CMV-Cer-FL- Bmal L95E

    Plasmid

    #47374

    Purpose

    Bmal full length with cer tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    L95E

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pCDNA3-HA/Bmal F423R/V435R/W427R

    Plasmid

    #47351

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    F423R/V435R/W427R

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

35. HLH Clock Pas 1-401 VenN L113E

    Plasmid

    #47356

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, L113E

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

36. HLH Clock Pas 1-401 VenN F122D

    Plasmid

    #47357

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, F122D

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

37. HLH Clock Pas 1-401 VenN W284A

    Plasmid

    #47358

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, W284A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

38. HLH Clock Pas 1-401 VenN V315R

    Plasmid

    #47359

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, V315R

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

39. HLH Bmal Pas 1-465-VenC L115E

    Plasmid

    #47377

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    L115E

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

40. HLH Clock Pas 1-401 VenN L57E

    Plasmid

    #47354

    Purpose

    Clock fragment mutatation tagged with VenN

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, L57E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

41. pBABE-puro-THAP11-rescue-3xFLAG

    Plasmid

    #36998

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 (THAP11 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Mutations were generated at codons 273-275 conver…

    Promoter

    Available since

    May 21, 2012

    Availability

    Academic Institutions and Nonprofits only

42. Magoh198delG

    Plasmid

    #30481

    Depositor

    Debra Silver, William Pavan

    Article

    Silver et al Nat Neurosci. 2010 May . 13(5):551-8.

    Insert

    Magoh (Magoh Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    198delG (point nucleotide deletion)

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

43. pRK5-HA-Ubiquitin-K48

    Plasmid

    #17605

    Purpose

    Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48 only, other lysines mutated to arginines. Enh…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRK5-HA-Ubiquitin-K63

    Plasmid

    #17606

    Purpose

    Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K63 only, other lysines mutated to arginines. Enh…

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pT7-PE7 for IVT

    Plasmid

    #214813

    Purpose

    Template for in vitro transcription of PE7

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. EGFP-CAAX

    Plasmid

    #86056

    Purpose

    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Homo sapiens Harvey rat sarcoma (HRAS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains the region from 825-857 of HRAS as a far…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pIDK-HDAC1fl

    Plasmid

    #109158

    Purpose

    Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. NA20 LN16

    Plasmid

    #101870

    Purpose

    Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

    Depositor

    Paul Clapham

    Article

    Peters et al J Virol. 2004 Jul;78(13):6915-26.

    Insert

    Env (env )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

49. pcDNA 3.3 LRP5

    Plasmid

    #115907

    Purpose

    Expresses human LRP5 in mammalian cells.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    LRP5 (LRP5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pSLIK 3xFLAG-MAVS hygro

    Plasmid

    #158634

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge