Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,931 results for: gast

Showing: 2981 - 3000 of 3931 results

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pRP[Exp]-CAG>dme_path[NM_001274705.1](ns):3xGGGGS:EGFP

Plasmid

#248611

Purpose

C-terminal GFP tag for GOI

Depositor

Lawrence Reiter

Insert

path (path Fly)

Tags

eGFP

Expression

Mammalian

Available since

Aug. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pRP[Exp]-CAG>dme_Pcmt[NM_080508.4](ns):3xGGGGS:EGFP

Plasmid

#248612

Purpose

C-terminal GFP tag for GOI

Depositor

Lawrence Reiter

Insert

pcmt (Pcmt Fly)

Tags

eGFP

Expression

Mammalian

Available since

Aug. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pRP[Exp]-CAG>dme_eIF6[NM_145105.3](ns):3xGGGGS:EGFP

Plasmid

#248601

Purpose

C-terminal GFP tag for GOI

Depositor

Lawrence Reiter

Insert

eIF6 (eIF6 Fly)

Tags

eGFP

Expression

Mammalian

Available since

Aug. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pRP[Exp]-CAG>dme_janA[NM_176585.2](ns):3xGGGGS:EGFP

Plasmid

#248605

Purpose

C-terminal GFP tag for GOI

Depositor

Lawrence Reiter

Insert

janA (janA Fly)

Tags

eGFP

Expression

Mammalian

Available since

Aug. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-d5-HT2BR

Plasmid

#221827

Purpose

Plasmid to express gRNA1 (ttcagtttgcccggtttaac) for editing at the end of Drosophila 5-HT2BR coding frame

Depositor

Quentin Gaudry

Publication

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2BR gRNA1 (CG42796 Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-d5-HT2BR

Plasmid

#221828

Purpose

Plasmid to express gRNA2 (aggcactcgtgctcgaatag) for editing at the end of Drosophila 5-HT2BR coding frame

Depositor

Quentin Gaudry

Publication

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

d5-HT2BR gRNA2 (CG42796 Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA1-for-dSERT

Plasmid

#221830

Purpose

Plasmid to express gRNA1 (cgaaatctgcgctctacttg) for editing at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Publication

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

dSERT gRNA1 (SerT Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pdU6-2_sgRNA2-for-dSERT

Plasmid

#221831

Purpose

Plasmid to express gRNA2 (gggattcgagcggcccgtcg) for editing at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Publication

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

dSERT gRNA2 (SerT Fly)

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6 promoter

Available since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

Plasmid

#221838

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Publication

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-spatzle-HA

Plasmid

#240235

Purpose

Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

spatzle (spz Fly)

Tags

HA-spGFP11

Expression

Insect

Promoter

UAS

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Tudor isoform A (HK130)

Plasmid

#206465

Purpose

Tudor expression in Schneider cells for imaging

Depositor

Mandy Jeske

Publication

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Tudor (tud Fly)

Tags

PH mcherry

Expression

Insect

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-NOT3 isoform A (HK100)

Plasmid

#206457

Purpose

NOT3 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Publication

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT3 (Not3 Fly)

Tags

PH mcherry

Expression

Insect

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-NOT3 isoform A (HK55)

Plasmid

#206442

Purpose

NOT3 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Publication

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT3 (Not3 Fly)

Tags

mEGFP

Expression

Insect

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-NOT2 isoform A (HK99)

Plasmid

#206456

Purpose

NOT2 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Publication

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

NOT2 (Rga Fly)

Tags

PH mcherry

Expression

Insect

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMtnA FLAG-IntS7 puro

Plasmid

#208405

Purpose

Expresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Publication

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

pMT FLAG MCS puro (defl Fly)

Tags

3xFLAG

Expression

Insect

Promoter

MtnA

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMtnA FLAG-IntS13 puro

Plasmid

#208406

Purpose

Expresses FLAG-tagged Drosophila IntS13 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Publication

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS13 (asun Fly)

Tags

3xFLAG

Expression

Insect

Promoter

MtnA

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-del331-370_AH

Plasmid

#148911

Purpose

Insect Expression of DmGIGYF-del331-370

Depositor

Elisa Izaurralde

Publication

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370 (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF-del331-370_AH

Plasmid

#148921

Purpose

Insect Expression of DmGIGYF-del331-370

Depositor

Elisa Izaurralde

Publication

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370 (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-GGSG-DmBam_13-36_AG

Plasmid

#148827

Purpose

Insect Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Publication

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Insect

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1467-1717_AE

Plasmid

#148598

Purpose

Insect Expression of DmNot1_1467-1717

Depositor

Elisa Izaurralde

Publication

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmNot1_1467-1717 (Not1 Fly)

Expression

Insect

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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