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pHIE061 ZF43x6-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138742

Purpose

Integration Vector, assembled with pHIE045 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE065 ZF43x12-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138746

Purpose

Integration Vector, assembled with pHIE049 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE059 ZF43x1 mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138740

Purpose

Integration Vector, assembled with pHIE043 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x1

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138741

Purpose

Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138744

Purpose

Integration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138745

Purpose

Integration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIE062 ZF43x12-C mKate2 EF1a EBFP2-P2A-BlastR

Plasmid

#138743

Purpose

Integration Vector, assembled with pHIE046 (TUPV1), pJM451 (TUPV2), pPD612

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Compact

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

Plasmid

#121804

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75246

Purpose

CRISPR/Cas9 NICKASE plasmid against human Helios (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75247

Purpose

CRISPR/Cas9 NICKASE plasmid against human Helios (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge