We narrowed to 4,850 results for: U6...

1. pORANGE Cloning template vector

   Plasmid

   #131471

   Purpose

   Cloning template form ORANGE method based knock-in constructs

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. v3em-Cterm-PE2max-∆RNaseH-dualU6

   Plasmid

   #198735

   Purpose

   AAV genome encoding C-terminal PE2max and U6 expression cassettes

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   NpuC-CtermPE2max∆RNaseH
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cbh

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL3.7-TNFa-shRNA3

   Plasmid

   #72598

   Purpose

   Express shRNA against mouse TNF-alpha

   Depositor

   Kazuhiro Oka

   Article

   Ogawa et al PLoS One. 2014 Mar 18;9(3):e92073. doi:

   Insert

   mouse TNF-alpha shRNA (Tnf Mouse)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Jan. 11, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. gRNA-CasRx_SD1-pSV40-TagBFP

   Plasmid

   #221004

   Purpose

   Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   CasRx 30nt processed direct repeat with SD1 mutation
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   DR1 A>T

   Promoter

   U6

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-Tet1-CD

   Plasmid

   #83340

   Purpose

   To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

   Depositor

   Ronggui Hu

   Article

   Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

   Insert

   dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   U6, CBH

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNEU-hMbPylRS-4xU6M15

   Plasmid

   #105830

   Purpose

   Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

   Depositor

   Irene Coin

   Article

   Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

   Inserts

   Humanized MbPylRS
   tRNAM15
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (Y271A, Y349F)MbPylRS

   Promoter

   CMV and U6

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA_526

   Plasmid

   #184859

   Purpose

   Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Feb. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiGuide-Neo

   Plasmid

   #139449

   Purpose

   Lentiviral vector with gRNA scaffold and neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   no sgRNA inserted; resistance gene: neoR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-dCas9-SSAP-MS2-BB_BbsI

   Plasmid

   #183826

   Purpose

   pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   U6-guideRNA-dCas9-T2A-EBFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWN_U6-TRAC-miniGag-Cas9

    Plasmid

    #228959

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPUR-mU6-sgRNA-2XPP7

    Plasmid

    #194504

    Purpose

    Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWN_U6-B2M-miniGag-Cas9

    Plasmid

    #228958

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE497

    Plasmid

    #153249

    Purpose

    M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGE332

    Plasmid

    #153241

    Purpose

    M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGE333

    Plasmid

    #153242

    Purpose

    M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGE335

    Plasmid

    #153244

    Purpose

    M3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGE337

    Plasmid

    #153246

    Purpose

    M4E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTE4398

    Plasmid

    #74042

    Purpose

    Expresses human codon-optimized LbCpf1 and Lb crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Lb crRNA
    LbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-Sp-pegRNA-RNF2_+5GtoT

    Plasmid

    #135957

    Purpose

    S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    RNF2_5GtoT pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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