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pIVT-mRuby2-P2A-PuroR_pKG02755

Plasmid

#246371

Purpose

IVT template for making modRNA encoding mRuby2-P2A-PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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Multi-Janus-Spacer-1x_pKG1126

Plasmid

#246375

Purpose

Multi-Janus spacer for Galloway Lab Golden Gate cloning scheme. Used as a "filler" plasmid in transfection experiments.

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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TET-ZF37-mCherry-BGH_pKG01502

Plasmid

#246377

Purpose

ZFa Plasmid expressing VP16-ZF37-2A-mCherry from the TET promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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TET-ZF43-TagBFP-BGH_pKG01531

Plasmid

#246378

Purpose

ZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the TET promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_EF1a-VP16-ZF43-2A-TagBFP-BGH_pKG2942

Plasmid

#246346

Purpose

ZFa Lentivirus expressing VP16-ZF43-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_EF1a-VP16-ZF37-2A-mCherry-BGH_pKG2943

Plasmid

#246347

Purpose

ZFa Lentivirus expressing VP16-ZF37-2A-mCherry from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_EF1a-VP16-ZF37-2A-TagBFP-WPRE_pKG3675

Plasmid

#246348

Purpose

ZFa Lentivirus expressing VP16-ZF37-2A-TagBFP from the EF1a promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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LentiX_PGK-VP16-ZF37-2A-TagBFP-WPRE_pKG3958

Plasmid

#246349

Purpose

ZFa Lentivirus expressing VP16-ZF37-2A-TagBFP from the hPGK promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-TagBFP

Use

Lentiviral and Synthetic Biology

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-VP16-ZF37-2A-mCherry-WPRE_pKG2987

Plasmid

#246350

Purpose

ZFa Retrovirus expressing VP16-ZF37-2A-mCherry

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Retroviral

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-VP16-ZF43-2A-TagBFP-WPRE_pKG2988

Plasmid

#246351

Purpose

ZFa Retrovirus expressing VP16-ZF43-2A-TagBFP

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Retroviral

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-GIB-Cre-N-WPRE_pKG3943

Plasmid

#246352

Purpose

GIB-Cre Retrovirus expressing N-terminal giberellin inducible Cre

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

iCre-N229-L1-GID1-NLS

Use

Retroviral

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-GIB-Cre-C-WPRE_pKG3944

Plasmid

#246353

Purpose

GIB-Cre Retrovirus expressing C-terminal giberellin inducible Cre

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

GAI-L1-iCre-230C-NLS

Use

Retroviral

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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(loxPcon-DIAL-YB_TATA)-mGL-BGH_pKG2787

Plasmid

#246327

Purpose

loxPcon DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of ZFa

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHR Myc-Oskar-FusionRed-FRB-iLID

Plasmid

#223289

Purpose

The Oskar germ granule protein tagged with optogenetic and chemogenetic tags for recruitment of desired cargo. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al Nat Commun. 2024 Aug 7;15(1):6717. doi:

Insert

myc-Oskar-FusionRed-FRB-iLID

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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DoS1Δinc

Plasmid

#244464

Purpose

DoS1 derived plasmid without RP4 inc

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

mCherry (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

pLtetO

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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DoS1ΔoriT

Plasmid

#244465

Purpose

DoS1 derived plasmid without RP4 oriT

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

mCherry (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

pLtetO

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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DoS1

Plasmid

#244463

Purpose

Containing RP4 oriT and inc sequence, for eliminating RP4 plasmid.

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

mCherry (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

pLtetO

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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DoS1ΔincΔoriT

Plasmid

#244466

Purpose

DoS1 derived plasmid without RP4 oriT and inc

Depositor

Lingchong You

Article

Tsoi et al Sci Adv. 2025 Apr 4;11(14):eads4735. doi

Insert

mCherry (CSS3 Synthetic)

Use

Synthetic Biology

Promoter

pLtetO

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBD-PYR1-MANDI

Plasmid

#241279

Purpose

Yeast two hybrid vector expressing BD-PYR1-MANDI

Depositor

Sean Cutler

Article

Park et al Nature. 2015 Apr 23;520(7548):545-8. doi

Insert

PYR1_mandi (PYR1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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MBP-Streptavidin-RGG

Plasmid

#242451

Purpose

Expresses the MBP-Streptavidin-RGG fusion protein in E Coli.

Depositor

Benjamin Schuster

Article

Kelley et al Nat Commun. 2025 Apr 14;16(1):3521. doi:

Insert

MBP-Streptavidin-RGG (using LAF-1 RGG domain)

Tags

MBP and Steptavidin

Expression

Bacterial

Available Since

Sept. 5, 2025

Availability

Academic Institutions and Nonprofits only

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