...Description Category PI Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP...pAAV-CAG-GFP CAG GFP Control Boyden 50469 pAAV-CaMKIIa-EGFP CamKIIa EGFP Control Roth 59462 pAAV-CAG-tdTomato...pAAV-CAG-tdTomato CAG tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2...-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP...-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG...pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson 114469 pAAV-CaMKIIa-mCherry CamKIIa mCherry Control Deisseroth 114470...pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 117382 hSyn1-eYFP Syn EYFP Control Gradinaru 135630 pAAV-S5E2...

...pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid...SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag Control for cell...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid ...CLIP-tag Control for mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-tag Control for cell...pMCP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101131 pACP-GPI Control Plasmid...-tag Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag Control for cell...mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization...

...in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element Gordon Fishell 83894...in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element Gordon Fishell 83895...

...-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control Zeng 50465 pAAV-hSyn-EGFP...pAAV-hSyn-EGFP Syn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKII EGFP Control Roth 114472 pAAV-hSyn-...pAAV-hSyn-mCherry Syn mCherry Control Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Deisseroth 50459...mCherry, Cre-dependent Control Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden 50457 pAAV-hSyn-DIO-EGFP...Cre-dependent Control Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Deisseroth... Cre-dependent Control Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth 55641...EYFP, Flp-dependent Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 114471 ...

...pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng....LacZ.bGH Control James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. ...eGFP.WPRE.rBG Control James M. Wilson AV-1-PV1963 105542-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG Control James M....pENN.AAV.CMVs.TurboRFP.WPRE.RBG Control James M. Wilson AV-1-PV2642 105552-AAV1 pENN.AAV.hSyn.TurboRFP.WPRE.RBG Control James M...eYFP.WPRE.hGH Control Karl Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James....SV40 Control Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren ....EGFP.WPRE.bGH Control James M. Wilson AV-2-27056 27056-AAV2 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth...

...CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that...76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441...,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled...76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,...,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled...57,050 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. 57,050 ...18,901 human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition...

...specificity control, secondary antibody specificity control, label control, and cross-reactivity control when...helpful to focus on something controllable, like experiments (and their controls!). This blog post will discuss... ins and outs of controls for biological experiments, starting with general controls and then moving on... of controls and be able to use this information to design thoughtfully and thoroughly controlled experiments...experiments.  Broadly useful controls Positive and negative controls Most biological experiments require...positive and negative controls to ensure proper interpretation of results. A positive control is generally a ...serum or isotype-specific control (Hewitt 2014, Alexander 2018). In this control, the primary antibody is...

... undergo stringent quality control by Addgene or a trusted quality control partner before distribution...Addgene's antibody production methods and quality control processes for purified recombinant antibodies. ...production protocols, titering methods, and quality control are described below. Information for a specific...samples with >90% purity are distributed. Quality Control Each lot of antibody undergoes testing, either ...fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections are...Protein expression is compared to an untransfected control and the protein size is confirmed. Residual Plasmid...testing for loss of potency against a non-treated control in one of its recommended assays. If an antibody...

...Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview of...subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma The 293T...Addgene's viral production methods and quality control for lentivirus and AAV. Viral...virus preps are titered and subjected to quality control by Addgene before being distributed to customers...production protocols, titering methods, and quality control are described below. AAV Production AAV distributed...probes targeting the ITR elements and an internal control of known titer (protocol modified from Lock et ...performed by droplet digital PCR (ddPCR). Quality Control Addgene ensures high quality viral vectors by optimizing...

...negative control. The negative control may be a no-antibody control in which a sample is processed without ...standard curve can serve as the positive control); and a negative control, a sample known not to contain your...interest. The negative control should be biological (no protein expressed), which controls for your antibody...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and a negative...addition to the control used in standard IP, ChIP should additionally use a beads-only control which runs ...the machine. Controls for cell sorting methods Flow cytometry requires a negative control, a small subset...basic steps needed for each application, important controls, and any special considerations to think about...

...replicate controls.  Transfection controls: empty vector and internal control A transfection control measures...Negative controls: untreated cells, empty vector control, and non-targeting control Negative control conditions...lacking the proper controls are invariably inconclusive and unreliable. Proper controls provide the constant...about the various controls that can be used for plasmid-based experiments. What are control plasmids? Generally...experiment, delineate its requisite control plasmids, and discuss why these control plasmids are critical for the...use of proper control plasmids would address and answer these questions. Types of control plasmids Part...the target cells. One transfection control is an empty vector control; specifically, the plasmid without...

...loading control is not perturbed by the experiment The loading control is supposed to control for, well...western blots benefit from a positive control — a loading control — that you know is present in every lane...transfer. Figure 1: A loading control is a type of positive control necessary to demonstrate a POI’s...loading control: the intensity of each POI band is divided by the intensity of the loading control in the...with loading controls. Under each lane, the fraction of the POI band over the loading control is shown. ...loading control. First, let’s explore why these assumptions are key to using loading controls accurately...opposite change in your loading control. Assumption #2: The loading control can be accurately quantified ...

...familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as an...nonspecific binding. Flow specific controls The three types of flow cytometry controls we’ll discuss are single ... may need to include additional controls like FMO or isotype controls, which we’ll describe a bit later...color control for every fluorophore you intend to use in your panel. Using single color controls On a ...your single color controls may not be accurate for your samples. The single color controls should be used...plots for a single color control. A) A flow plot showing a single color control for the APC channel. B)...likely to require FMOs. Isotypes Isotype controls are negative controls that allow you to determine what level...

...Optogenetics plasmids encode proteins that are controlled by light. Many of these plasmids encode for microbial...the cell. When using these opsins as a tool to control neuronal activity, scientists generally attach ...localization. Many other types of proteins can also be controlled by light, generally by utilizing a light sensitive...fusions can then be used as an optical switch to control protein interactions, dimerization, localization...the table below for plasmids that use light to control a protein or cellular activity. For example, search...

...non-standard), expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Gradinaru 103006...(non-standard), expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Gradinaru 127847...non-standard), expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Gradinaru 175004...plasmid (non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Gradinaru 175005...plasmid (non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Gradinaru 185136...non-standard), expressing Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Gradinaru 185137...non-standard), expressing Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Gradinaru All...

...contains a human codon optimized dcas9 gene under the control of Murine Stem Cell retroVirus LTR promoter. The...pgRNA_humanized ) contains a murine U6 promoter controlled sgRNA cassette, wherein the GN19 can be custom...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA...vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter 46921 pTDH3-dCas9-Mxi1 Yeast...and sgRNA targeting GAL4 UAS promoter (negative control) 46918 pU6-sgCD71-2 Human pSico-based U6 vector...Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1...Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE ...

... What kind of control? On, off, tunable, and/or inducible? How long do you need control? Will temporary...temporary control (a few days) be enough, or do you want a permanent control mechanism? Where is the control...vector! On/off control all the time If your experimental system requires tunable control - or maybe you...forever? You don’t need to resort to the force to control protein expression in mammalian cells: easy to ... will review tried-and-true protein expression control tools as well as explore some new innovations. ...temporarily, we’ve got you covered. Defining your control requirements To pick the best tool for the job,...protein expression. If you need the ability to control when you turn off expression for good, the Cre-...

...while pMB70 is designed for in vivo transcrition controlled by the regulatory sequences of an RNA polymerase...Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter. 45945 pHsp70-... : The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz,...BbsI-chiRNA : Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter. 51019...

...Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our control AAV that express... select plasmids in the repository. Browse our control AAV collection, including fluorescent proteins,...consider using fewer filtering options. Browse all Control AAV *AAVrg = retrograde serotype, produced with...

...DNA.  Plasmids are said to be under stringent control when they depend on the host cell’s initiation ...replication independently are said to be under relaxed control, as they only require the host's replication machinery...replication mechanism of plasmids under stringent control, such as pSC101. DNA replication and regulation...round of replication. Initiation is the primary control point of DNA replication in bacteria, so the process... the bacterial chromosome and for stringently controlled plasmids. Binding of IHF (green) redistributes...binding is necessary to initiate replication, controlling the amount of active DnaA available is one of...source, ATP binding is one more way for the cell to control DNA replication. A variety of other proteins also...