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We narrowed to 494 results for: control

Showing: 1 - 20 of 494 results

  1. A Control for All Seasons

    Type
    Blog Post
    ...specificity control, secondary antibody specificity control, label control, and cross-reactivity control when...helpful to focus on something controllable, like experiments (and their controls!). This blog post will discuss... ins and outs of controls for biological experiments, starting with general controls and then moving on... of controls and be able to use this information to design thoughtfully and thoroughly controlled experiments...experiments.  Broadly useful controls Positive and negative controls Most biological experiments require...positive and negative controls to ensure proper interpretation of results. A positive control is generally a ...serum or isotype-specific control (Hewitt 2014, Alexander 2018). In this control, the primary antibody is...

  2. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...replicate controls.  Transfection controls: empty vector and internal control A transfection control measures...Negative controls: untreated cells, empty vector control, and non-targeting control Negative control conditions...lacking the proper controls are invariably inconclusive and unreliable. Proper controls provide the constant...about the various controls that can be used for plasmid-based experiments. What are control plasmids? Generally...experiment, delineate its requisite control plasmids, and discuss why these control plasmids are critical for the...use of proper control plasmids would address and answer these questions. Types of control plasmids Part...the target cells. One transfection control is an empty vector control; specifically, the plasmid without...

  3. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...loading control is not perturbed by the experiment The loading control is supposed to control for, well...western blots benefit from a positive control — a loading control — that you know is present in every lane...transfer. Figure 1: A loading control is a type of positive control necessary to demonstrate a POI’s...loading control: the intensity of each POI band is divided by the intensity of the loading control in the...with loading controls. Under each lane, the fraction of the POI band over the loading control is shown. ...loading control. First, let’s explore why these assumptions are key to using loading controls accurately...opposite change in your loading control. Assumption #2: The loading control can be accurately quantified ...

  4. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as an...nonspecific binding. Flow specific controls The three types of flow cytometry controls we’ll discuss are single ... may need to include additional controls like FMO or isotype controls, which we’ll describe a bit later...color control for every fluorophore you intend to use in your panel. Using single color controls On a ...your single color controls may not be accurate for your samples. The single color controls should be used...plots for a single color control. A) A flow plot showing a single color control for the APC channel. B)...likely to require FMOs. Isotypes Isotype controls are negative controls that allow you to determine what level...

  5. Caltech Systemic Capsids

    Type
    Collection
    ...Description Category PI Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP...pAAV-CAG-GFP CAG GFP Control Boyden 50465 pAAV-hSyn-EGFP hSyn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP...pAAV-CaMKIIa-EGFP CamKIIa EGFP Control Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell...-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP...pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH...pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson 114469 pAAV-CaMKIIa-mCherry...pAAV-CaMKIIa-mCherry CamKIIa mCherry Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 117382 ...

  6. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid...SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag Control for cell...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid ...CLIP-tag Control for mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-tag Control for cell...pMCP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101131 pACP-GPI Control Plasmid...-tag Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag Control for cell...mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization...

  7. Brain Initiative Collection

    Type
    Collection
    ...in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element Gordon Fishell 83894...in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element Gordon Fishell 83895...

  8. Retrograde AAV viral preps

    Type
    Collection
    ...-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control Zeng 50465 pAAV-hSyn-EGFP...pAAV-hSyn-EGFP Syn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKII EGFP Control Roth 114472 pAAV-hSyn-...pAAV-hSyn-mCherry Syn mCherry Control Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Deisseroth 50459...mCherry, Cre-dependent Control Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden 50457 pAAV-hSyn-DIO-EGFP...Cre-dependent Control Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Deisseroth... Cre-dependent Control Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth 55641...EYFP, Flp-dependent Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 114471 ...

  9. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng....LacZ.bGH Control James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. ...eGFP.WPRE.rBG Control James M. Wilson AV-1-PV1963 105542-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG Control James M....pENN.AAV.CMVs.TurboRFP.WPRE.RBG Control James M. Wilson AV-1-PV2642 105552-AAV1 pENN.AAV.hSyn.TurboRFP.WPRE.RBG Control James M...eYFP.WPRE.hGH Control Karl Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James....SV40 Control Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren ....EGFP.WPRE.bGH Control James M. Wilson AV-2-27056 27056-AAV2 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth...

  10. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... What kind of control? On, off, tunable, and/or inducible? How long do you need control? Will temporary...temporary control (a few days) be enough, or do you want a permanent control mechanism? Where is the control...vector! On/off control all the time If your experimental system requires tunable control - or maybe you...forever? You don’t need to resort to the force to control protein expression in mammalian cells: easy to ... will review tried-and-true protein expression control tools as well as explore some new innovations. ...temporarily, we’ve got you covered. Defining your control requirements To pick the best tool for the job,...protein expression. If you need the ability to control when you turn off expression for good, the Cre-...

  11. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...DNA.  Plasmids are said to be under stringent control when they depend on the host cell’s initiation ...replication independently are said to be under relaxed control, as they only require the host's replication machinery...replication mechanism of plasmids under stringent control, such as pSC101. DNA replication and regulation...round of replication. Initiation is the primary control point of DNA replication in bacteria, so the process... the bacterial chromosome and for stringently controlled plasmids. Binding of IHF (green) redistributes...binding is necessary to initiate replication, controlling the amount of active DnaA available is one of...source, ATP binding is one more way for the cell to control DNA replication. A variety of other proteins also...

  12. Lentiviral Prep Service

    Type
    Collection
    ...CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that...76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441...,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled...76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,...,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled...57,050 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. 57,050 ...18,901 human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition...

  13. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...light-based control of signaling pathways. This is particularly exciting because using light to control signaling...  Option #2 may sound like fiction, but light-controlled protein purification is now a reality thanks ..., and their use for protein purification and controlling cell signaling. Key components of OptoNBs OptoNBs...insertion into loops 1 and 6 resulted in light controlled binding, but with opposite effects: insertion...insertion site on light-induced binding.  Light controlled protein purification with OptoNBs The first application...application for OptoNBs takes us in vitro: light-controlled affinity purification of unmodified proteins....stuck to the surface of a bead.  Using OptoNBs to control cell signaling The second application for OptoNBs...

  14. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...viral prep pAAV-Ef1a-fDIO EYFP AAV5. AAV8, AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG...pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237) AAV-PHPeB Control Jonathan Ting New viral prep AiP12787 - pAAV-...minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) AAV-PHPeB Control Jonathan Ting New viral prep AiP12610 - pAAV-...pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) AAV-PHPeB Control Jonathan Ting New viral prep AiP13755: pAAV-AiE0743m...10aa-H2B-WPRE3-BGHpA (Alias: CN4496) AAV-PHPeB Control Jonathan Ting New viral prep AiP14825: pAAV-AiE0441h...prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiSSTe10_ChR2... pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiCHATe27_...

  15. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...Noninvasive Controlling Stimulation ExogenousEasily controlled EndogenousNot easily controlled Unique...spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad control of whole...boundaries in neuroscience by granting targeted control over neuronal activity. While they serve similar...optogenetics differ are: Timing Targeted manipulation Controlling stimulation Invasiveness  Choosing the best ...Chemogenetics does not offer the same level of control over timing. Because chemogenetics relies on chemical... can reach target cells throughout the body. Controlling Stimulation Some studies rely on delivering reversible...stimulation to receptors. Optogenetics offers better control over these factors as the light source can be easily...

  16. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...concepts discussed in the paper. The transcriptional control of multiple loci is deftly coordinated by the eukaryotic...tinkering with regulatory networks and multiplex gene control. However, these approaches are often met with the...Modifying the CRISPRi system for multidirectional control The CRISPR interference (CRISPRi) model consists...CRISPRi system for more flexible multidirectional control, a new study repurposes the sgRNA to include modular...The use of regulators allows a greater degree of control of desired expression levels dependent upon the...of simultaneous and multidirectional multiplex control via scRNA design is readily apparent in metabolic...elegantly demonstrated the potential of this to control a metabolic network in S. cerevisiae by using three...

  17. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...is sufficient. Controls: Controls can make or break an experiment. The best controls for a colony PCR... They also serve as controls for your PCR reaction. Running a no template control PCR reaction for detecting...bacterial debris. Use a positive control. A good positive control is bacteria transformed with the same...primer design. Use a negative control strain. A good negative control strain is an untransformed culture...type of control is especially important for insert-specific primers. If your negative control amplifies...number of background colonies on your no ligation control plate. The more background, the more colonies you...backbone vector with and without an insert. These controls are quick references you can use when you run ...

  18. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...an appropriate control? Many CRISPR screens use non-targeting guides as negative controls. Non-targeting...are well characterized and strategies exist to control for these faults. However, it’s still unclear if...screens and how best to design these screens to controls for flaws. Recently the Bassik Lab at Stanford...site in the genome. This means that while they control for many of the effects of gRNA and Cas9 expression...detecting Cas9 cutting. Safe targeting guides better control for non-specific toxicity than non-targeting guides... a coding region. Targeting these sites should control for the effects of guide expression and dsDNA breaks... the genome, and are commonly used as negative controls for genome-wide CRISPR screens; and 3) safe-targeting...

  19. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...tracking for instance) can be controlled with FLP-FRT. For further temporal control, Tamoxifen inducible Cre...system, but it only gives you a single level of control without modification - either Cre is there or it...for site specific (and often cell type specific) control of DNA recombination and gene expression can be...addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed. Read...FLP and Cre enable additional levels of genetic control. What is FLP FRT and how does it work? FLP recombinase...expressing the light activatable ion channel under the control of Cre recombinase are used in combination with...occur at a specific time point? To allow temporal control, ligand regulated Cre and FLPe recombinase have...

  20. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...and other proteins controlled by GAL4 and ZIF268. To demonstrate independent control of gene expression...a GAL4/estradiol controlled transcription factor and a ZIF268/progesterone controlled transcription factor...transcription factor 1 controls expression of transcription factor 2, which directly controls expression of a...chemically inducible approaches to provide temporal control, this new system, Fkbp/Frb Inducible Recruitment...PubMed PMID: 28916764. Plasmids for independent control of gene expression in yeast Article contributed...yeast transcription factors that can independently control expression of your genes of interest. These transcription...possible to use synthetic transcription factors to control the level of random variation, or noise, in reporter...
Showing: 1 - 20 of 494 results