...Description Category PI Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP...pAAV-CAG-GFP CAG GFP Control Boyden 50469 pAAV-CaMKIIa-EGFP CamKIIa EGFP Control Roth 59462 pAAV-CAG-tdTomato...pAAV-CAG-tdTomato CAG tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2...-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP...-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG...pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson 114469 pAAV-CaMKIIa-mCherry CamKIIa mCherry Control Deisseroth 114470...pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 117382 hSyn1-eYFP Syn EYFP Control Gradinaru 135630 pAAV-S5E2...

...pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B Control Plasmid...SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag Control for cell...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid ...CLIP-tag Control for mitochondrial localization 101125 pCLIPf-NK1R Control Plasmid CLIP-tag Control for cell...pMCP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101131 pACP-GPI Control Plasmid...-tag Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag Control for cell...mammalian expression 101133 pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization...

...in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element Gordon Fishell 83894...in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element Gordon Fishell 83895...

...-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent Control Zeng 50465 pAAV-hSyn-EGFP...pAAV-hSyn-EGFP Syn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKII EGFP Control Roth 114472 pAAV-hSyn-...pAAV-hSyn-mCherry Syn mCherry Control Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Deisseroth 50459...mCherry, Cre-dependent Control Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control Boyden 50457 pAAV-hSyn-DIO-EGFP...Cre-dependent Control Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Deisseroth... Cre-dependent Control Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth 55641...EYFP, Flp-dependent Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 114471 ...

...pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-1-ALL854 51502-AAV1 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng....LacZ.bGH Control James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. ...eGFP.WPRE.rBG Control James M. Wilson AV-1-PV1963 105542-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG Control James M....pENN.AAV.CMVs.TurboRFP.WPRE.RBG Control James M. Wilson AV-1-PV2642 105552-AAV1 pENN.AAV.hSyn.TurboRFP.WPRE.RBG Control James M...eYFP.WPRE.hGH Control Karl Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG Control James....SV40 Control Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren ....EGFP.WPRE.bGH Control James M. Wilson AV-2-27056 27056-AAV2 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth...

...CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that...76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441...,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled...76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,...,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled...57,050 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. 57,050 ...18,901 human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition...

... undergo stringent quality control by Addgene or a trusted quality control partner before distribution...Addgene's antibody production methods and quality control processes for purified recombinant antibodies. ...production protocols, titering methods, and quality control are described below. Information for a specific...samples with >90% purity are distributed. Quality Control Each lot of antibody undergoes testing, either ...fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections are...Protein expression is compared to an untransfected control and the protein size is confirmed. Residual Plasmid...testing for loss of potency against a non-treated control in one of its recommended assays. If an antibody...

...Production Titer Quality Control Resources Lentivirus Production Titer Quality Control Resources Overview of...subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma The 293T...Addgene's viral production methods and quality control for lentivirus and AAV. Viral...virus preps are titered and subjected to quality control by Addgene before being distributed to customers...production protocols, titering methods, and quality control are described below. AAV Production AAV distributed...probes targeting the ITR elements and an internal control of known titer (protocol modified from Lock et ...performed by droplet digital PCR (ddPCR). Quality Control Addgene ensures high quality viral vectors by optimizing...

...negative control. The negative control may be a no-antibody control in which a sample is processed without ...standard curve can serve as the positive control); and a negative control, a sample known not to contain your...interest. The negative control should be biological (no protein expressed), which controls for your antibody...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and a negative...addition to the control used in standard IP, ChIP should additionally use a beads-only control which runs ...the machine. Controls for cell sorting methods Flow cytometry requires a negative control, a small subset...basic steps needed for each application, important controls, and any special considerations to think about...

...Optogenetics plasmids encode proteins that are controlled by light. Many of these plasmids encode for microbial...the cell. When using these opsins as a tool to control neuronal activity, scientists generally attach ...localization. Many other types of proteins can also be controlled by light, generally by utilizing a light sensitive...fusions can then be used as an optical switch to control protein interactions, dimerization, localization...the table below for plasmids that use light to control a protein or cellular activity. For example, search...

...non-standard), expressing Rep2 and PHP.eB Cap controlled by tTA-TRE amplification system Gradinaru 103006...(non-standard), expressing Rep2 and PHP.S Cap controlled by tTA-TRE amplification system Gradinaru 127847...non-standard), expressing Rep2 and PHP.V1 Cap controlled by tTA-TRE amplification system Gradinaru 175004...plasmid (non-standard), expressing Rep2 and B10 Cap controlled by tTA-TRE amplification system Gradinaru 175005...plasmid (non-standard), expressing Rep2 and B22 Cap controlled by tTA-TRE amplification system Gradinaru 185136...non-standard), expressing Rep2 and MaCPNS1 Cap controlled by tTA-TRE amplification system Gradinaru 185137...non-standard), expressing Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Gradinaru All...

...contains a human codon optimized dcas9 gene under the control of Murine Stem Cell retroVirus LTR promoter. The...pgRNA_humanized ) contains a murine U6 promoter controlled sgRNA cassette, wherein the GN19 can be custom...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA...vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter 46921 pTDH3-dCas9-Mxi1 Yeast...and sgRNA targeting GAL4 UAS promoter (negative control) 46918 pU6-sgCD71-2 Human pSico-based U6 vector...Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1...Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE ...

...while pMB70 is designed for in vivo transcrition controlled by the regulatory sequences of an RNA polymerase...Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter. 45945 pHsp70-... : The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz,...BbsI-chiRNA : Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter. 51019...

...Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse our control AAV that express... select plasmids in the repository. Browse our control AAV collection, including fluorescent proteins,...consider using fewer filtering options. Browse all Control AAV *AAVrg = retrograde serotype, produced with...

...Ready-to-use AAV available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype ...list of all 100 µL sized sample, please see our Control AAV Inventory . The catalog ID for the 20 µL sized... catalog item, including the titer and quality control, visit the material page (linked for each catalog...

... in search of inducible promoters capable of controlling eukaryotic gene expression. Several endogenous... regulator of gene expression, a tetracycline-controlled transactivator (tTA) was developed. tTA was created... transactivator rtTA ( r everse t etracycline-controlled t rans a ctivator) was created by fusing rTetR...Puro_Tet3G_3xFLAG_Twin_Strep Bidirectional promoter controls expression of gene of interest with Strep-Tag ...Blast (w756-1) Lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV...and an inducible promoter at its 3' end, which controls GFP expression tTA Off Verma 26803 pEnt L1L3 EF1a-tTA...enhancer options tTA Off Uney References Tight control of gene expression in mammalian cells by tetracycline-responsive...

...Engineering Visualization and Tagging Purification Controlled Expression Reporter Plasmids Reporter Plasmids...Plasmids Tagging and Visualization Purification Controlled Expression Reporter Plasmids You may also like… ...Manipulating the genome directly can give you more subtle control over protein expression and activity thereby limiting...protein’s function under a wide range of highly controlled conditions in vitro , or, if you want to determine...can be made and tested at once. Return to Top Controlled Expression It isn’t always beneficial to constitutively...stationary phase. In these cases you may want to control protein levels or to turn on expression only at...expression levels have been well studied or can be controlled by small molecules, light, temperature, or even...

.... Addgene’s comprehensive high-quality quality control process includes titering, purity assessment, sterility... Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster, Brainbow...Serotype Serotype Testing Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1...

...targeted neuronal manipulation (aka intersectional control). The end result of the meeting will be a publicly...tips for using the technology in the lab (e.g., controls to perform, recommended histology conditions, ... examples of AAV strategies for intersectional control. Please see our list of suggested materials below...reference for AAV use in neuronal intersectional control. Through your participation in this meeting, you...

...facilitation, DNA amplification, and high-quality quality control measures such as titering, purity assessment, sterility...centrifugation purification Addgene’s comprehensive quality control process: Titering Purity assessment Sterility testing...produce the prep, and perform comprehensive quality control processes to make sure it is ready for your experiments...including purification, titration, and quality control measures. We have distributed preps around the ...