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We narrowed to 29 results for: control

Showing: 1 - 20 of 29 results

  1. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...and FAQ Do controls When doing ligations you should ALWAYS do a vector alone + ligase control. This will...The following table indicates the various controls: Control Ligase Interpretation Uncut vector - Checks...know has only been thawed once before. Always do controls. See Tips and FAQ below for details. Try different...treated, that the phosphatase treatment worked. This control should, in principle, be free of colonies, but ...colonies than your vector alone ligation. Additional controls are encouraged, but may only be required for troubleshooting...

  2. Colony Formation Titering Assay

    Type
    Protocol
    ...titer of your lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...All of the cells in the untransduced (negative) control well should be killed and no colonies should be...appropriate antibiotic. When the untransduced (negative control) cells have died and colonies are visible in the...

  3. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...insert. It is also important to set up negative controls in parallel. For instance, a ligation of the recipient...plasmid alone plate. The recipient plasmid alone control will tell you your “background” level or more specifically...see any colonies, you should conduct a positive control to ensure that your transformation worked. You ...depending on the number of background colonies on your control plate (the more background, the more colonies you...

  4. Fluorescence Titering Assay

    Type
    Protocol
    ... titer of your lentiviral vector allows you to control the multiplicity of infection (MOI) in downstream...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Inverted fluorescence...dilution with one well remaining as the untransduced control). Count the cells in the remaining well, a cell...

  5. Protocol - Bacterial Transformation

    Type
    Protocol
    ...should also add a positive control (many companies include a positive control plasmid with their competent...ligation of inserts to vectors than for an intact control plasmid. Incubate the competent cell/DNA mixture...

  6. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...insert. It is also important to set up negative controls in parallel. For instance, a ligation of the recipient...plasmid alone plate. The recipient plasmid alone control will tell you your “background” level or more specifically...see any colonies, you should conduct a positive control to ensure that your transformation worked. You ...depending on the number of background colonies on your control plate (the more background, the more colonies you...

  7. Immunocytochemistry

    Type
    Protocol
    ...Day 3-4: Fix and label cells Equipment Pipette controller Pipette tips and pipettes Rocking platform Tweezers...functioning as expected and specific, include a positive control sample that you know expresses the protein, such...express the protein of interest, and a negative control sample such as cells that do not express the protein...

  8. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Multichannel pipette reagent reservoirs Pipette controller Microcentrifuge Microplate shaker ELISA plate... antigen Purified recombinant antibody Isotype control antibody HRP-conjugated isotype-specific secondary...mix. Dilute the primary antibody and an isotype control antibody to the desired concentrations in the antibody...recombinant purified Desmin (blue) or a negative control protein, Apolipoprotein L3 (red) overnight, blocked...

  9. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...plate will hold 5 dilutions plus one 'no virus' control well). Perform a "reverse transduction" by seeding...selection should last at least as long as it takes the control (untransduced) cells to completely die. After that...

  10. Western Blot

    Type
    Protocol
    ...pipette 200–1000 µL single channel pipette Pipette controller Pipette tips and pipettes Spectrophotometer Heat...as expected and is specific, include a positive control sample that you know expresses the protein, such...transfected with the protein of interest, and a negative control sample that does not express the protein of interest...

  11. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...express Cas9 and are included as a control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift...µL single channel pipette CO 2 incubator Pipet controller Hazardous waste container Microscope Hemocytometer...

  12. Lentivirus ddPCR Titration

    Type
    Protocol
    ...ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control for normalization. The dilution series outlined...wells containing the virus and the untransduced control. Pro-Tip For even seeding, prepare a batch for ...concentration of RRE positive droplets in the untransduced control should be close to zero (A01). In this protocol...

  13. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...samples being tested. Always include a No Template Control (NTC), i.e master mix + water Whenever possible...duplicate. Do not forget to include a no template control ( NTC = master mix + water ). Add 15 μL of Master...

  14. Kit Free RNA Extraction

    Type
    Protocol
    ...unlike using a p1000 tip it will give you more control of where you’re aiming your tip in the tube. Precipitate...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing non-hairpin insert. .... pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid... parallel. This plate will serve as a positive control for the puromycin selection. Days 4+: g. Change...

  20. Centrifugation

    Type
    Protocol
    ...the rotor with microfuge tubes in place, and the control panel where you can set the time and speed needed...
Showing: 1 - 20 of 29 results