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We narrowed to 11,455 results for: ARIA

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Showing: 4501 - 4530 of 11455 results

  2. pET19-TdTc253

    Plasmid
    #207464
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    MTdTc253 (Dntt Mouse)
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207464-plasmid-map-sequence-id-407817

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  3. pET19-TdTc188

    Plasmid
    #207463
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    TdTc188 (Dntt Mouse)
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Cys216Ser, Cys302Ala, Cys378Ala, and Cys438Ser
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207463-plasmid-map-sequence-id-407799

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  4. pET19-TdTc180

    Plasmid
    #207462
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    TdTc180 (Dntt Mouse)
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Glu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207462-plasmid-map-sequence-id-407791

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  5. pET19-TdTc302

    Plasmid
    #207461
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    TdTc302 (Dntt Mouse)
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207461-plasmid-map-sequence-id-407790

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  9. pcDNA-GFP-EBA175-Flag(E1418K/P1424Q/Y1426S)

    Plasmid
    #155006
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/P1424Q/Y1426S; insert codes for EBA175 res…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155006-plasmid-map-sequence-id-309986

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  10. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H)

    Plasmid
    #155313
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    M1423R/P1424V/Y1425W/Y1426H; insert codes for EBA…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155313-plasmid-map-sequence-id-310080

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  11. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1426H)

    Plasmid
    #155312
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    M1423R/P1424V/Y1426H; insert codes for EBA175 res…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155312-plasmid-map-sequence-id-309984

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  12. pcDNA-GFP-EBA175-Flag(A1419R/P1424Q/Y1426S)

    Plasmid
    #155007
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    A1419R/P1424Q/Y1426S; insert codes for EBA175 res…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155007-plasmid-map-sequence-id-309775

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  13. pcDNA-GFP-EBA175-Flag(S1421R/P1424Q/Y1426S)

    Plasmid
    #155008
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    S1421R/P1424Q/Y1426S; insert codes for EBA175 res…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155008-plasmid-map-sequence-id-309834

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  14. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S)

    Plasmid
    #155009
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/A1419R/P1424Q/Y1426S; insert codes for EBA…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155009-plasmid-map-sequence-id-309962

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  15. pcDNA-GFP-BAEBL (I1134V/P1135L/Y1136V/F1137V)

    Plasmid
    #154875
    Purpose
    Expresses N-terminally GFP-tagged Pf-BAEBL P5-P2 VLVV variant (coding for residues 1041-1210) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfBAEBL (PF3D7_0506900 P. falciparum)
    Tags
    GFP and signal peptide (residues 1 to 32) from TG…
    Expression
    Mammalian
    Mutation
    I1134V, P1135L, Y1136V, F1137V; insert codes for …
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154875-plasmid-map-sequence-id-310429

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  16. pcDNA-GFP-EBA175-Flag(P1424I/Y1425I/Y1426I)

    Plasmid
    #154977
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424A/Y1425I/Y1426I variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424/Y1425I/Y1426I; insert codes for EBA175 resi…
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154977-plasmid-map-sequence-id-309851

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  17. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139988-plasmid-map-sequence-id-275338

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  19. pCKC775

    Plasmid
    #226626
    Purpose
    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, sfGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    7xTCF-LEF and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226626-plasmid-map-sequence-id-449161

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  20. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT S. pyogenes, Chicken, Human)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

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  21. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Promoter
    4xHRE_YB TATA and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  22. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  25. USPL1cat-C236S-pEYFP_C1

    Plasmid
    #85767
    Purpose
    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236S
    Depositor
    Frauke Melchior
    Article
    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10
    Insert
    USPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    catalytic domain (aa 212-514), catalytic inactiv…
    Promoter
    CMV
    Available Since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85767-plasmid-map-sequence-id-169512

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  26. pETM6-3F2-mCherry

    Plasmid
    #73418
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73418-plasmid-map-sequence-id-135318

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  27. pETM6-3A2-mCherry

    Plasmid
    #73425
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3A2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3A2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73425-plasmid-map-sequence-id-135325

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  28. pETM6-5F5-mCherry

    Plasmid
    #73422
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 5F5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P5F5 (orthogonal T7-lac variant)
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73422-plasmid-map-sequence-id-135322

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Showing: 4501 - 4530 of 11455 results