We narrowed to 3,760 results for: biorxiv
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Plasmid#236150PurposeExpress mutated UTRN-14 zinc finger array (N62K, H89F, I91E, K121F) attached by a deimmunized linker to the NZF transcriptional activation under control of CMV promoter with two TetR binding sitesDepositorInsertUTRN-14ZF-deImmLink-NZF
UseTagsExpressionMammalianMutationN61K, H88F, I91E, K120F in UTRN-14ZFPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW446 CMV-TO-ADAR2dd(E488Q, T501A) (FLP-IN)
Plasmid#236125PurposePlasmid encoding free ADAR2dd under control of CMV promoter with two TetR binding sitesDepositorInsertADAR2dd (ADARB1 Synthetic, Human)
UseTagsExpressionMammalianMutationE488Q, T501APromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF1 CMV-TO-SCN1A-377(G2E, A104K, S109I)-deImmLink-NZF
Plasmid#236153PurposeExpress mutated SCN1A-14 zinc finger array (G2E, A104K, S109I) attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-377-deImmLink-NZF
UseTagsExpressionMammalianMutationG2E, A104K, S109I in SCN1A-377PromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
KL094 - pTRE3G-Flag-Halo-CTCF_WT-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor
Plasmid#232930PurposeVector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF WT, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.DepositorInsertFlag-Halo-CTCF WT
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mCherry)
Plasmid#233542PurposeA CMV promoter driving expression of a murine dominant negative myc tagged TCF4.DepositorInsertTCF4 (TCF4 Mouse)
UseLentiviralTagsMycExpressionMutationlacks aa 1-56PromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG
Plasmid#200793PurposeMammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutation1-914; Δ505-610PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TMEM16A-I547K-peGFP-N1
Plasmid#228583PurposeExpresses mouse TMEM16A-I547K-eGFP in mammalian cellsDepositorInsertTMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
UseTagsExpressionMammalianMutationI547KPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TMEM16A-E551K-peGFP-N1
Plasmid#228584PurposeExpresses mouse TMEM16A-E551K-eGFP in mammalian cellsDepositorInsertTMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)
UseTagsExpressionMammalianMutationE551KPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB1_v1.3 (MTK 3a) (pZYW044)
Plasmid#194227PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB1DepositorInsertLCB1_v1.3
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB3_v1.2 (MTK 3a) (pZYW045)
Plasmid#194228PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB3DepositorInsertLCB3_v1.2
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
WPRE (MTK 4b) (pAN2410)
Plasmid#194235PurposeMammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbonesDepositorInsertWPRE terminator
UseSynthetic BiologyTagsExpressionMutationPromoterNoneAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9
Plasmid#223163Purpose2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7-S72A
Plasmid#221556PurposeExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationChange serine 72 to alaninePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)
Plasmid#221552PurposeExpresses Cas9 and gRNA for disruption of Rab7A gene in human cellsDepositorInsertgRNA targeting human Rab7A (RAB7A Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa VP64 Hbb
Plasmid#99693PurposeExpresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Synthetic, Mouse)
UseAAV and CRISPRTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITESceIcmd
Plasmid#218288PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1DepositorInsertHO(-253, -1)-tSynth3UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC3.1_CAG_oROS-Gr_LF(C199S)
Plasmid#216114PurposeExpresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.DepositorInsertoROS-Gr_LF(C199S)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-TEV-NAP1
Plasmid#217610PurposeFor expression in E. coli of NAP1DepositorInsertNAP1 (AZI2 Human)
UseTagsGSTExpressionBacterialMutationPromoterTAC promoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-TEV-GFP-NAP1
Plasmid#208870PurposeFor expression in E. coli of GFP-NAP1DepositorInsertNAP1 (AZI2 Human)
UseTagsGFP and GST-TEVExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC3 CTF-FLAG
Plasmid#217686PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR3 (CELSR3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2516PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only