We narrowed to 15,088 results for: pET

1. pET23b-Casp3 C163A-His

   Plasmid

   #11822

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 3 catalytic mutant (CASP3 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C163A

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pET_His6-SUMO-TEV-TtCsm6

   Plasmid

   #172486

   Purpose

   Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

   Insert

   T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
   

   Tags

   His-SUMO-TEV

   Expression

   Bacterial

   Promoter

   T7 promoter and lac operator

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a(+) NRP1 b1 (273-427)

   Plasmid

   #162734

   Purpose

   Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

   Depositor

   Brett Collins

   Article

   Daly et al Science. 2020 Oct 20. pii: science.abd30

   Insert

   Human NRP1 b1 domain residues 273-427
   

   Expression

   Bacterial

   Mutation

   Codon optimised

   Available Since

   April 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET41-MBP-t-A3A-t-His

   Plasmid

   #109231

   Purpose

   Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

   Depositor

   Rahul Kohli

   Article

   Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

   Insert

   human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)
   

   Tags

   His and MBP

   Expression

   Bacterial

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-26b-NbVHH05-HA-His

   Plasmid

   #171567

   Purpose

   Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-HA-His (UBE2J1 Human)
   

   Tags

   HA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET19b-rDAD2 cPGFP(G164)

   Plasmid

   #165994

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rDAD2 cPGFP(G164)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-TEX264-deltaLRR-His6

   Plasmid

   #220215

   Purpose

   Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   c.4-75del / p.2-24del

   Promoter

   T7

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-AviTag-DogTag-MBP

   Plasmid

   #171773

   Purpose

   Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   AviTag-DogTag-MBP
   

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG94-1--LaG94-14

   Plasmid

   #228973

   Purpose

   For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-1--LaG94-14
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-21a(+)-AK1-His

    Plasmid

    #118977

    Purpose

    Bacterial expression plasmid for Adenylate kinase isoenzyme 1

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22b-mEB3-eGFP-6xHis

    Plasmid

    #130983

    Purpose

    Bacterial expression of mouse EB3-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB3-GFP-6xHis (Mapre3 Mouse)
    

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a_DmKHC[1-421]-1xGFP-6xHis

    Plasmid

    #196972

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    6xHis and GFP

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Duet-1-NONO(53-312)

    Plasmid

    #135442

    Purpose

    Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    Non-POU domain-containing octamer-binding protein (NONO Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. WT C-His Ana GvpC in pET28a

    Plasmid

    #85732

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-SENP2 (catalytic domain)

    Plasmid

    #16357

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP2 catalytic domain (SENP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 365 to 590 catalytic domain of human S…

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET_MBP-RGG-GFP-RGG

    Plasmid

    #124949

    Purpose

    Expression of MBP-RGG-GFP-RGG

    Depositor

    Daniel Hammer, Benjamin Schuster, Matthew Good

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    MBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    EGFP, His, and MBP

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-His6-GFP-C-LACE

    Plasmid

    #133913

    Purpose

    Bacterial expression of His6-GFP-C-LACE

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    superfolder green fluorescent protein
    

    Tags

    LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21 Catalytic Domain of MALT1

    Plasmid

    #48970

    Purpose

    Expresses catalytic domain of MALT1 in e.coli AA 329-566

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 (MALT1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains AA 329-566

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pET-15b PAI-RBP

    Plasmid

    #45390

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP (SERBP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET23b-2E2-HA-mEGFP-6xHis

    Plasmid

    #129594

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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