We narrowed to 14,886 results for: pET

1. pET28-sfGFP-DogTag Loop A

   Plasmid

   #171779

   Purpose

   Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   sfGFP-DogTag Loop A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   DogTag and linkers inserted between residues Val2…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-MHL: nsp16_SARS-CoV-2

   Plasmid

   #167850

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp16

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp16
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET28-MHL: nsp10_SARS-CoV-2

   Plasmid

   #167851

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp10

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pET21a-GFP-AURKA_K162M-mCherry

   Plasmid

   #99881

   Purpose

   Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Mutation

   K162M (kinase-dead)

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6xHis-(pos9)GFP

   Plasmid

   #89247

   Purpose

   Expresses GFP with +9 theoretical charge in E.Coli

   Depositor

   David Liu

   Article

   Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

   Insert

   His6-(pos9)-GFP
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SENP7 (catalytic domain)

   Plasmid

   #16360

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP7 catalytic domain (SENP7 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 640 to 984 catalytic domain of human S…

   Promoter

   Available since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pET28a-2xMreBN-mCherry-His

   Plasmid

   #133626

   Purpose

   Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   2xMreB-mCherry-His
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET15b-Casp8 delta DED

   Plasmid

   #11827

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 8 minus Death Effecter Domain (CASP8 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Minus Death Defecter Domain (aa 1-216)

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

9. 1a. pETM11-SUMO-SNCA-GFP

   Plasmid

   #107292

   Purpose

   Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-GFP (SNCA Human)
   

   Use

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-Sac-Abeta(MC1-40)

    Plasmid

    #127152

    Purpose

    Expresses N-terminal cysteine Abeta(1-40)

    Depositor

    James Nowick

    Insert

    Abeta(MC1-40) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET19b-EcMscL-TEV-mEGFP

    Plasmid

    #165097

    Purpose

    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

    Depositor

    Neha Kamat

    Article

    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

    Insert

    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    

    Use

    Synthetic Biology

    Tags

    monomeric Enhanced Green Fluorescent Protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-SUMO-hDDI2-D252N_pET11a

    Plasmid

    #169122

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D252N

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-51b(+)-cpHaloTag141/145

    Plasmid

    #194660

    Purpose

    Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHaloTag141/145
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-51b(+)-cpHaloTag154/156

    Plasmid

    #194661

    Purpose

    Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHaloTag154/156
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-link-ymYPET-URA3

    Plasmid

    #168056

    Purpose

    Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymYPET
    

    Use

    Tags

    ymYPET

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Mucin MUC1-5TR-pET15b

    Plasmid

    #174694

    Purpose

    Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

    Depositor

    Jean Gariepy

    Article

    Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

    Insert

    MUC1-TR
    

    Use

    Tags

    hexahistidine (HIS) tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET24a-VHH-tev-mCherry

    Plasmid

    #117752

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET22-Nsp10-TEV-6His

    Plasmid

    #154957

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pET28a-SARS-CoV-2 NSP13

    Plasmid

    #176492

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

    Depositor

    Xiaodong Cheng

    Article

    White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

    Insert

    SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    I570 from SARS-CoV NSP13 was mutated to V to conv…

    Promoter

    T7

    Available since

    Jan. 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. His6-SUMO-pET28a-EfaCas1

    Plasmid

    #112793

    Purpose

    To express enterococcus faecalis Cas1 protein in E. coli

    Depositor

    Ailong Ke

    Article

    Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

    Insert

    EfaCas1
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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