We narrowed to 15,088 results for: pET

1. pET28-6His-SUMO-Nsp10

   Plasmid

   #154959

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET28_mouse-Caspase-1_enzymatic-domain

   Plasmid

   #183385

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 (AA130-404) (Casp1 Mouse)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-Synapsin-YPET-C1-WPRE

   Plasmid

   #159954

   Purpose

   untargeted marker

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   YPET-C1
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-His6MBP-A3A-Gly6His6

   Plasmid

   #187822

   Purpose

   Expression human APOBEC3A in E.coli.

   Depositor

   Hao Wu

   Article

   Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

   Insert

   APOBEC3A (APOBEC3A Human)
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 promoter (on the backbone)

   Available Since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-MBP-NLS-Geo_st

   Plasmid

   #87703

   Purpose

   Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

   Depositor

   Jennifer Doudna

   Insert

   GeoCas9
   

   Tags

   10xHis-MBP-TEV

   Expression

   Bacterial

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a SUMO Nup53xl 1-320

   Plasmid

   #85998

   Purpose

   bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   Xenopus laevis Nup53 (nup35.L Frog)
   

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-24a-ELP[V-150]

   Plasmid

   #67015

   Purpose

   Expresses ELP[V-150] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   ELP[V-150]
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-21a-PE2-His

   Plasmid

   #201973

   Purpose

   Express His-tagged PE2 in bacteria

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   PE2-HIS
   

   Tags

   His and c-myc NLS

   Expression

   Bacterial

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-EIN-AlkBH5(66-292)

   Plasmid

   #125732

   Purpose

   Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

   Depositor

   Vincenzo Venditti

   Article

   Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

   Insert

   AlkBH5(66-292) (ALKBH5 Human)
   

   Tags

   6xHis and EIN

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-His6-Ran(Q69L)

    Plasmid

    #171563

    Purpose

    Encodes human Ran protein

    Depositor

    Yan Zeng

    Insert

    Ran (RAN Human)
    

    Expression

    Bacterial

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-6xHis-(pos16)GFP

    Plasmid

    #89249

    Purpose

    Expresses GFP with +16 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30a-His6-ABP-LOXCAT

    Plasmid

    #139971

    Purpose

    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b SARS-CoV PLpro

    Plasmid

    #61693

    Purpose

    inducible expression of SARS PLpro protease

    Depositor

    Michael Johnson

    Article

    Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

    Insert

    SARS-CoV PLpro
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pET-21a(+)/MBP-His

    Plasmid

    #38006

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    MBP (Maltose binding protein)
    

    Tags

    6xHIS

    Expression

    Bacterial

    Available Since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pET30-ARH3-His-Sumo-HA

    Plasmid

    #111578

    Purpose

    Expresses ARH3 (amino acids 14-363) in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    ARH3 (ADPRS Human)
    

    Tags

    6xHis-Sumo and HA

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-13

    Promoter

    T7

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b-ACPs (from S. pneumonia)

    Plasmid

    #63687

    Purpose

    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae

    Depositor

    Michael Johnson

    Insert

    acpS
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pET28_human-Caspase-4_enzymatic-domain

    Plasmid

    #183381

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (AA94-377) (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28sumo-MDA5-Full-length

    Plasmid

    #210826

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

    Insert

    MDA5 (IFIH1) Full Length Protein (IFIH1 Human)
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b-ptetO::bac::gfp

    Plasmid

    #217882

    Purpose

    expresses the bac-BGC in bacterial cells, produces bacillamide D

    Depositor

    Tobias A. M. Gulder

    Article

    Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

    Inserts

    Dehydrogenase
    Non-ribosomal peptide synthetase
    Aminotransferase
    

    Expression

    Bacterial

    Promoter

    ptetO

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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