We narrowed to 5,756 results for: cki

1. pSEED-mGreenLantern:ALFA

   Plasmid

   #219644

   Purpose

   Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mGreenLantern:ALFA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ecoli cpGFP

   Plasmid

   #215713

   Purpose

   Expresses cpGFP in Ecoli

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   circularly permuted GFP

   Promoter

   T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-TurboID-NES_pLX304

   Plasmid

   #202008

   Purpose

   expresses TurboID in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-TurboID-NES
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

   Plasmid

   #31484

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   EF1s HA NLS Sce(opt) T2A IFP1.4
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   "Opt" indicates the I-SceI coding seque…

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEED

   Plasmid

   #219658

   Purpose

   Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Empty SEED Cassette (3xP3-dsRed)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LeGO-EBFP2

   Plasmid

   #85213

   Purpose

   Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   EBFP2
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEED-sfGFP

   Plasmid

   #219639

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mini-donor - SOX17-EGFP

    Plasmid

    #206048

    Purpose

    this mini-donor knockin vector can be used to knock EGFP into SOX17 locus

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    nuclear EGFP
    

    Use

    Knockin donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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