We narrowed to 5,876 results for: nls

1. TDP-43 _CTD_6F→A

   Plasmid

   #194269

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→A

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Synthetic, Human)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   F276A, F283A, F289A, F367A, F397A, F401A

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TDP-43 _CTD_5M→A_CR

   Plasmid

   #194263

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CR

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Synthetic, Human)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   M322A, M323A, M336A, M337A, M339A mutations in TD…

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TDP-43 _CTD_5M→A_IDR

   Plasmid

   #194264

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to Alanine

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Synthetic, Human)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   M307A, M311A, M359A, M405A, M414A mutations in TD…

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TDP-43 _CTD_5M→L_IDR

   Plasmid

   #194265

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucine

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   M307L, M311L, M359L, M405L, M414L mutations in TD…

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TDP-43 _CTD_5M→Y_IDR

   Plasmid

   #194266

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosine

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Synthetic, Human)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   M307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TDP-43 _CTD_5A→M_IDR

   Plasmid

   #194267

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionine

   Depositor

   Nicolas Fawzi

   Article

   Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Insert

   TDP-43 (TARDBP Synthetic, Human)
   

   Use

   Tags

   6XHistag

   Expression

   Bacterial

   Mutation

   A297M, A367M, A381M, A388M, A391M mutations in TD…

   Promoter

   T7

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBridge-tyrosinase.σ3A

   Plasmid

   #197513

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-3 σ3A
   

   Use

   Tags

   GAL4-DNA binding domain fragment, HA tag, and nuc…

   Expression

   Yeast

   Mutation

   silent substitution in codon 2 of tyrosinase tail…

   Promoter

   ADH1 and MET25

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sniper2P

   Plasmid

   #193857

   Purpose

   Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

   Insert

   Sniper2L
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   E1007L

   Promoter

   EFS

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHEN-CoV2-nanobody-C7

   Plasmid

   #194591

   Purpose

   Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

   Insert

   C7 (S )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pC1-ND-Cdt1-mCitrine

    Plasmid

    #193771

    Purpose

    Human Cdt1 (SV40 NLS localization and C-terminal mCitrine tag) with mutations which abroggate its S phase degradation

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    ND-Cdt1-mCitrine (CDT1 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHA853 - mec-4p::Fynempty

    Plasmid

    #139210

    Purpose

    Control vector for Fyn kinase expression in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    deletion and frame shift to prevent Fyn expression

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHA851 - osm-10p::Fynempty

    Plasmid

    #139208

    Purpose

    Control vector for Fyn kinase expression in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    deletion and frame shift to prevent Fyn expression

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUS_LC_charge_RtoK

    Plasmid

    #139127

    Purpose

    express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Add K and D to FUS LC so it has the same charge p…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FUS_LC_charge

    Plasmid

    #139126

    Purpose

    express FUS LC with an hnRNPA2 LC-like charge patterning

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Add R, K, D to make FUS LC have similar charge pa…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHJ4

    Plasmid

    #158785

    Purpose

    Pan-neuronal nuclear-localized GCaMP expression in C. elegans

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Prab-3::nls::GCaMP6m
    

    Use

    Tags

    NLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hnRNPA2_LC_WT_S285C

    Plasmid

    #98665

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S285C

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hnRNPA2_LC_D290V_S285C

    Plasmid

    #98666

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S285C and D290V

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S285C D290V

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hnRNPA2_LC_WT_S329C

    Plasmid

    #98667

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with S329C

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S329C

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hnRNPA2_LC_D290V_S329C

    Plasmid

    #98668

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V and S329C

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D290V S329C

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hnRNPA2_LC_D290V

    Plasmid

    #98658

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341 with D290V

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D290V

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge