We narrowed to 7,077 results for: GFP expression plasmids

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pAAV-Syn-FLEX-rc [ChromeQ-GFP]

Plasmid

#153540

Purpose

AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeQ-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC2234 - pAAV Nestin GFP-iCre

Plasmid

#228443

Purpose

An adeno-associated viral vector to express GFP-iCre fusion protein under the human Nestin 2nd intron enhancer.

Depositor

Christopher Richie

Article

Ribeiro Gomes et al Cell Rep. 2025 Dec 21;45(1):116756. doi:

Insert

GFP-iCre

Use

AAV

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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rAAV-Ef1α-EGFP-WPRE-ID-PolyA

Plasmid

#241829

Purpose

AAV construct for constitutive expression of EGFP. Contains a molecular barcode for identification of AAV genome using single-cell sequencing.

Depositor

Benjamin Arenkiel

Article

Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

Insert

EGFP-WPRE-ID-PolyA

Use

AAV

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [ChromeT-GFP]

Plasmid

#153539

Purpose

AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97309

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-0.27kb-EGFP

Plasmid

#153185

Purpose

Truncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-0.66kb-EGFP

Plasmid

#153165

Purpose

Truncated mouse gamma-synuclein (mSncg-0.66kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-1.03kb-EGFP

Plasmid

#153164

Purpose

Truncated mouse gamma-synuclein (mSncg-1.03kb) promoter-mediates gene expression in mammalian retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJET-42-DExCon-antiGFPnanobody-mCherry-R11a

Plasmid

#179902

Purpose

Donor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

antiGFPnanobody-mCherry

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJRH-1328 LV EF1a-CD20 IRES-EGFP

Plasmid

#201918

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD20

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138133

Purpose

Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)

Plasmid

#126026

Purpose

express RFP-tagged LARIAT variant for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHRolig-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

Plasmid

#136627

Purpose

Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-R59

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP

Plasmid

#32476

Purpose

Expresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAM Y115F,L141F-5HT3 HC

Tags

IRES-GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

Plasmid

#199346

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126024

Purpose

express RFP-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HFW-CRY2-VHH(GFP)

Plasmid

#126027

Purpose

express Flag-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Tags

Flag

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT7R-7xsfGFP11-HA-HDR-donor

Plasmid

#221818

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT7R coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT7R homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor

Plasmid

#221832

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1AR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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