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pMSCV-NINJ1

Plasmid

#201179

Purpose

Expression of NINJ1 gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Use

Retroviral

Tags

Flag

Promoter

MESV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Plasmid

#158967

Purpose

Ubiquitous expression of mtON in C. elegans

Depositor

Andrew Wojtovich

Article

Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

Insert

eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

Tags

GFP

Expression

Worm

Promoter

eft-3

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJH4783

Plasmid

#200785

Purpose

Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

snb-1 (snb-1 Nematode)

Tags

pHiuorin

Expression

Worm

Promoter

Pnpr-4

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Cre stuffer v3

Plasmid

#158030

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV-IRES-GFP-human-Caspase-1

Plasmid

#183359

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (CASP1 Human)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

Plasmid

#129774

Purpose

Marker of microtubule dynamics in C. elegans hypodermis

Depositor

Andrew Chisholm

Article

Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

Inserts

Pcol-19
ebp-2 (ebp-2 Nematode)
unc-54 3'UTR

Use

Synthetic Biology; Gateway

Tags

Synthetic Intron and mGFP

Expression

Worm

Promoter

Pcol-19

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP8H

Plasmid

#188062

Purpose

PROSS-designed versatile peroxidase 8H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

38 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-rab9 DN

Plasmid

#12664

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

changed serine 21 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pMXs-OLIG2

Plasmid

#32933

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

OLIG2 (OLIG2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pET28_human-pro-IL18

Plasmid

#183390

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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REEP5-EGFP

Plasmid

#210363

Purpose

Expresses REEP5 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-PKCa-C1

Plasmid

#112264

Purpose

ITRACKalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase C alpha (PRKCA Bovine)

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-SYNDAPIN2

Plasmid

#221311

Purpose

Expression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

SYNDAPIN2 (pLxIS) (Pacsin2 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (wt)

Plasmid

#112799

Purpose

Expression of OCTN2

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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DsRed-rab9 DN

Plasmid

#12676

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 21 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-PAFAH1B1

Plasmid

#92430

Purpose

mammalian expression of PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN-HA

Plasmid

#130912

Purpose

Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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NSP3-FR-2Strep

Plasmid

#210315

Purpose

Expresses SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3 (ORF1ab SARS-CoV-2)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-Lhx3

Plasmid

#32927

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Lhx3 (Lhx3 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only