We narrowed to 3,095 results for: sam

1. pEGFP-Kif2c

   Plasmid

   #208651

   Purpose

   Mammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoter

   Depositor

   Samuel Bakhoum

   Article

   Li et al Nature. 2023 Aug;620(7976):1080-1088. do

   Insert

   Kif2c (KIF2C Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV hSyn INTRON HaloTag-Jph3 WPRE

   Plasmid

   #236238

   Purpose

   AAV expression of human Junctophilin3 N-terminally tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   Junctophilin 3 (JPH3 Human)
   

   Use

   AAV

   Tags

   HaloTag

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

   Plasmid

   #159630

   Purpose

   AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

   Depositor

   Ross Bathgate, Andrew Allen

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   Ast (AstA Fly)
   

   Use

   AAV

   Tags

   HA, IRES, mCherry, and neurophysin-1

   Expression

   Mammalian

   Promoter

   DCA (same as CAG promoter)

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-dnMCAK

   Plasmid

   #208650

   Purpose

   Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter

   Depositor

   Samuel Bakhoum

   Article

   Li et al Nature. 2023 Aug;620(7976):1080-1088. do

   Insert

   Kif2c (Kif2c Cricetulus griseus (Chinese hamster))
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   S92A , S106A , S108A, S112A , S186A, H530A, R534A…

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX304-DCK*-IRES-GFP

   Plasmid

   #176297

   Purpose

   Expresses DCK* and GFP from the same transcript

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Insert

   Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S74E R104M D133A

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304-DCK*-IRES-Td

   Plasmid

   #176298

   Purpose

   Expresses DCK* and TdTomato from the same transcript

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Insert

   Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S74E R104M D133A

   Promoter

   CMV

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-TRC.mKO2_shB3GNT5.1

   Plasmid

   #110325

   Purpose

   TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   B3GNT5 (B3GNT5 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307 hDVL1

   Plasmid

   #102863

   Purpose

   Expresses human DVL1 in mammalian cells

   Depositor

   Henry Ho

   Article

   Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

   Insert

   human Dishevelled 1 (DVL1) (DVL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Changed alanine 2 to glycine. Likely due to poly…

   Promoter

   EF1A

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-NAGK

   Plasmid

   #23785

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NAGK (NAGK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. 6MIW

    Plasmid

    #127291

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene

    Depositor

    Cheryl Arrowsmith

    Article

    Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    6xHis-TEV (MHHHHHHSSGRENLYFQG)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid

    #105805

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Venus

    Expression

    Mammalian

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

    Plasmid

    #105803

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-FLAG; ORF2: TEV-mClover3

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid

    #105806

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Amber

    Expression

    Mammalian

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV hSyn INTRON mEmerald-ER WPRE

    Plasmid

    #236230

    Purpose

    AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
    

    Use

    AAV

    Tags

    mEmerald

    Promoter

    human Synapsin 1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV hSyn INTRON HaloTag-Sec61b WPRE

    Plasmid

    #236229

    Purpose

    AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    HaloTag-Sec61b (SEC61B Human, Synthetic)
    

    Use

    AAV

    Tags

    HaloTag

    Promoter

    human Synapsin 1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid

    #236245

    Purpose

    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Promoter

    U6 and human Synapsin1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn INTRON mEmerald-Sec61b WPRE

    Plasmid

    #236228

    Purpose

    AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mEmerald-Sec61b (SEC61B Human, Synthetic)
    

    Use

    AAV

    Tags

    mEmerald

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pD649-HAsp-CLMP-COMP5AP-AviTag-9xHis

    Plasmid

    #157209

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CLMP (CLMP Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. RG6-BAP1_E7-D192D

    Plasmid

    #154024

    Purpose

    Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
    

    Use

    Splicing reporter

    Tags

    DsRed1, FLAG, SV40 NLS, and eGFP

    Expression

    Mammalian

    Mutation

    BAP1 c.576C>T, p.D192D

    Available Since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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