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pDEST15-Eps8L2_297-370

Plasmid

#196537

Purpose

Expression of GST-Eps8L2_297-370

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_297-370 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_367-496

Plasmid

#196538

Purpose

Expression of GST-Eps8L2_367-496

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_367-496 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2_494-546

Plasmid

#196539

Purpose

Expression of GST-Eps8L2_494-546

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_494-546 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-LASSIE

Plasmid

#192105

Purpose

Lentiviral vector expressing long non-coding RNA LASSIE (linc00520)

Depositor

Reinier Boon

Article

Stanicek et al Commun Biol. 2020 May 26;3(1):265. doi:

Insert

LASSIE

Use

Lentiviral

Promoter

CMV

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA-HA

Plasmid

#186374

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-HA-RfA

Plasmid

#186373

Purpose

Destination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA

Plasmid

#186370

Purpose

Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

Plasmid

#183387

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

redesigned Caspase-4 (AA94-377) (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

ANR261ENP, D315H, M318K, T352K

Promoter

T7

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pdestMB14_grh-1p-grh-1-gfp

Plasmid

#177761

Purpose

Overexpression of C. elegans grh-1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

grh-1 (grh-1 Nematode)

Expression

Worm

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-dre4

Plasmid

#170440

Purpose

Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

dre4 (dre4 Fly)

Tags

6xHis and V5

Expression

Bacterial

Mutation

Deleted amino acids 1-20

Promoter

T7

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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