We narrowed to 8,434 results for: Dos

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pATH (pKD-G6)

Plasmid

#62685

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP419/amiR-eGFP123

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pATK (pKD-G9)

Plasmid

#62688

Purpose

Mammalian expression of amiR-eGFP with a tdTomato gene from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP123/amiR-eGFP419

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pRevTre-mKLF5

Plasmid

#41744

Depositor

Jonathan Katz

Article

Yang et al Cell Cycle. 2012 Nov 1;11(21):4033-9. do

Insert

Klf5 (Klf5 Mouse)

Expression

Mammalian

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only
pET11+PCNA3-CYP119(D77R/T214V)

Plasmid

#66138

Purpose

Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1 H4 (haplotype 4)

Plasmid

#66718

Purpose

Constitutive expression of FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Gripentrog et al Immunogenetics. 2008 Feb;60(2):83-93. do

Insert

FPR1 (haplotype 4) (FPR1 Human)

Expression

Mammalian

Promoter

SR-alpha

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-PIM2mut2

Plasmid

#51850

Purpose

promoter region of PIM2 lacking the forkhead binding site #1

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

PIM2 (PIM2 Human)

Use

Luciferase; Reporter vector

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
UAS-dar1-V5

Plasmid

#45056

Depositor

Bing Ye

Article

Ye et al J Neurosci. 2011 Mar 2;31(9):3309-19. do

Insert

dar1 (dar1 Fly)

Tags

V5

Expression

Insect

Promoter

HS promoter

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only
Ecm2 1-143

Plasmid

#169923

Purpose

WT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2 1-143

Expression

Bacterial and Yeast

Availability

Academic Institutions and Nonprofits only

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Ecm2 1-265

Plasmid

#169925

Purpose

WT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2 1-265

Expression

Bacterial and Yeast

Availability

Academic Institutions and Nonprofits only

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Ecm2 1-325

Plasmid

#169926

Purpose

WT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2 1-325

Expression

Bacterial and Yeast

Availability

Academic Institutions and Nonprofits only

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pHH0103_PCMT1_PCMT

Plasmid

#109932

Purpose

Protein expression and purification of PCMT1_PCMT

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PCMT1_PCMT

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBa.KIF13B

Plasmid

#224413

Purpose

Mammalian expression of Kif 13b

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Kif 13b (Kif13b Mouse)

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE 3xHA-MKK6-EE puro

Plasmid

#216791

Purpose

Mammalian expression of constitutively active human MKK6

Depositor

Kevin Janes

Article

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Insert

MKK6 (MAP2K6 Human)

Use

Retroviral

Tags

3x-HA

Expression

Mammalian

Mutation

S207E and T211E, constitutively active

Promoter

LTR

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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GFAP-Lyn-mCherry-pHluorin

Plasmid

#187980

Purpose

Membrane targeted cytoplasmic fluorescent pH sensor

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Lyn-mcherry-pHluorin

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)

Plasmid

#104117

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

QuasAr3-dark Citrine

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pP2A-mCherry-N1

Plasmid

#84329

Purpose

Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

P2A-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-VSP4-E228Q

Plasmid

#80351

Purpose

Expression of GFP-tagged, dominant negative VPS4 mutant

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VPS4-E228Q (VPS4A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJWV102-PL-dCas9

Plasmid

#85588

Purpose

Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

dCas9sp

Use

CRISPR

Expression

Bacterial

Promoter

PLsp

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Expression

Bacterial

Promoter

PF6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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