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Showing: 3081 - 3100 of 31394 results

  6. B-HeFSpCas9

    Plasmid
    #126766
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126766-plasmid-map-sequence-id-259607

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  7. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

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  10. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid
    #101743
    Purpose
    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    FgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4, added 9F in FgßF3
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101743-plasmid-map-sequence-id-199091

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  14. B-eSpCas9

    Plasmid
    #126761
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126761-plasmid-map-sequence-id-259548

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  15. B-evoSpCas9

    Plasmid
    #126765
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126765-plasmid-map-sequence-id-410921

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Showing: 3081 - 3100 of 31394 results