We narrowed to 9,083 results for: mel

1. pAc5.1B-EGFP-DmTral_394-543-V5His6_D

   Plasmid

   #146134

   Purpose

   Insect Expression of DmTral_394-543

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral_394-543 (tral Fly)
   

   Expression

   Insect

   Mutation

   two silent mutations (nucleotides A198C, A387G) a…

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

   Plasmid

   #146071

   Purpose

   Insect Expression of DmTral-LSmR21EE23KR42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KR42EF44A (tral Fly)
   

   Expression

   Insect

   Mutation

   R21E, E23K, R42E, F44A. Additionally, one silent …

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-EGFP-DmTral-R42EF44A_C

   Plasmid

   #146061

   Purpose

   Insect Expression of DmTral-R42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42EF44A (tral Fly)
   

   Expression

   Insect

   Mutation

   R42E, F44A. Additionally, two silent mutations A1…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

   Plasmid

   #146049

   Purpose

   Insect Expression of DmTral-LSmR21EE23KS13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-LSmR21EE23KS13AI15A (tral Fly)
   

   Expression

   Insect

   Mutation

   S13A, I15A, R21E, E23K, one silent mutation A198C…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

   Plasmid

   #146039

   Purpose

   Insect Expression of DmTral-FDF-ADA

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmTral-FDF-ADA (tral Fly)
   

   Expression

   Insect

   Mutation

   FDF to ADA (407-409) mutation. Additionally, thre…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-chiRNA_dCrkgRNA1

   Plasmid

   #131139

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-chiRNA_dCrkgRNA2

   Plasmid

   #131140

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

   Plasmid

   #112987

   Purpose

   In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Mutation

   S123-125-3A

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D

   Plasmid

   #112985

   Purpose

   In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Mutation

   S123-125-3D

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL c2x Tral FL-15D

    Plasmid

    #113010

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to D

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

12. KHBD00320

    Plasmid

    #34445

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6376 (E2f1 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pLViN-E-cadherin-GFP

    Plasmid

    #229710

    Purpose

    Lentiviral expression of E-cadherin-GFPTG in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CDH1 E-CADHERIN Human (CDH1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-E55A-ATPIF1

    Plasmid

    #85404

    Purpose

    Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase

    Depositor

    David Sabatini

    Article

    Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

    Insert

    ATPIF1 (ATP5IF1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    E55A point mutation abrogating the ability of ATP…

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pUAS-ERK-YFP

    Plasmid

    #42076

    Depositor

    Norbert Perrimon

    Article

    Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

    Insert

    Drosophila ERK ortholog (rl Fly)
    

    Tags

    YFP

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

16. WT (DVD)

    Plasmid

    #184316

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid

    #131143

    Purpose

    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    mNeonGreen::3XFLAG::Crk (Crk Fly)
    

    Use

    Cre/Lox; Attb recombination site

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDam-Myc-HP1

    Plasmid

    #59221

    Purpose

    expresses Dam-myc-HP1

    Depositor

    Bas van Steensel

    Article

    van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

    Insert

    HP1 (Su(var)205 Fly)
    

    Use

    Damid

    Tags

    Dam and myc

    Expression

    Insect

    Promoter

    Drosophila heat shock promoter

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. KHBD00716

    Plasmid

    #39744

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17228 (pros Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pJC-mhc2.4kb-TALE3-VP64-P10

    Plasmid

    #104611

    Purpose

    Expresses TALE3 under the control of a 2.4kb mhc enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    2.4kb mhc enhancer element (Mhc Fly)
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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