We narrowed to 9,004 results for: mel

1. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

   Plasmid

   #146039

   Purpose

   Insect Expression of DmTral-FDF-ADA

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmTral-FDF-ADA (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   FDF to ADA (407-409) mutation. Additionally, thre…

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-chiRNA_dCrkgRNA1

   Plasmid

   #131139

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-chiRNA_dCrkgRNA2

   Plasmid

   #131140

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3A

   Plasmid

   #112987

   Purpose

   In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3A) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   S123-125-3A

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. mei-S332-GFP 5.6kb genomic DNA in Casper4 S123-125-3D

   Plasmid

   #112985

   Purpose

   In P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (S123-125-3D) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   S123-125-3D

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL c2x Tral FL-15D

   Plasmid

   #113010

   Purpose

   For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   15 PNG phosphorylation sites mutated to D

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc-GFP-dSUMOdeltaC

   Plasmid

   #85697

   Purpose

   expression of GFP-SUMO1 delta C in Drosophila cells

   Depositor

   Licio Collavin, Giannino Del Sal

   Article

   Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

   Insert

   SUMO (Sumo Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   non-conjugatable SUMO, lacking the C-terminal gly…

   Promoter

   pAc5

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

8. KHBD00320

   Plasmid

   #34445

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG6376 (E2f1 Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pLViN-E-cadherin-GFP

   Plasmid

   #229710

   Purpose

   Lentiviral expression of E-cadherin-GFPTG in mammalian cells

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CDH1 E-CADHERIN Human (CDH1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-E55A-ATPIF1

    Plasmid

    #85404

    Purpose

    Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase

    Depositor

    David Sabatini

    Article

    Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

    Insert

    ATPIF1 (ATP5IF1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    E55A point mutation abrogating the ability of ATP…

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pUAS-ERK-YFP

    Plasmid

    #42076

    Depositor

    Norbert Perrimon

    Article

    Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

    Insert

    Drosophila ERK ortholog (rl Fly)
    

    Use

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

12. WT (DVD)

    Plasmid

    #184316

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid

    #131143

    Purpose

    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    mNeonGreen::3XFLAG::Crk (Crk Fly)
    

    Use

    Cre/Lox; Attb recombination site

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDam-Myc-HP1

    Plasmid

    #59221

    Purpose

    expresses Dam-myc-HP1

    Depositor

    Bas van Steensel

    Article

    van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

    Insert

    HP1 (Su(var)205 Fly)
    

    Use

    Damid

    Tags

    Dam and myc

    Expression

    Insect

    Mutation

    Promoter

    Drosophila heat shock promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. KHBD00716

    Plasmid

    #39744

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17228 (pros Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pJC-mhc2.4kb-TALE3-VP64-P10

    Plasmid

    #104611

    Purpose

    Expresses TALE3 under the control of a 2.4kb mhc enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    2.4kb mhc enhancer element (Mhc Fly)
    

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. KHBD00474

    Plasmid

    #39584

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17077 (pnt Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pAc5.1B-EGFP-DmeIF4E

    Plasmid

    #79258

    Purpose

    Expresses EGFP-tagged DmeIF4E in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

    Insert

    eIF4E (eIF4E1 Fly)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUAST-HA, Pbl-A PH+DH domains

    Plasmid

    #69793

    Purpose

    Pebble isoform A, PH and DH domains, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    van Impel et al Development. 2009 Mar;136(5):813-22. doi

    Insert

    Pebble DH +PH domains (pbl Fly)
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA tag

    Expression

    Insect

    Mutation

    Pbl-A (NP_729306.1) amino acids 385-647.

    Promoter

    hsp70 promoter

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A_R

    Plasmid

    #147389

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A (gw Fly, Nematode)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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